Optimization of Chlorella Culture Conditions with Response Surface Methodology to Increase Biomass

Microalgae is gaining popularity as a major ingredient in nutrition supplements. To mass cultivate, it is imperative to improve the biomass yield hence optimization of cultures conditions becomes paramount. In this work, an attempt has been made to optimize the microalgal production using response s...

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Bibliographic Details
Published in:Nature environment and pollution technology 2021-12, Vol.20 (5), p.2111-2116
Main Authors: Kanimozhi, R., Prasath, D. Arvind, Dhandapani, R., Sigamani, Santhosh
Format: Article
Language:English
Subjects:
Algae
Aquatic microorganisms
Biomass
Carbon
Chlorella
Cyanobacteria
Design of experiments
Dietary supplements
Glycerol
Light
Light intensity
Luminous intensity
Nutrition
Optimization
Response surface methodology
response surface methodology, biomass, optimization, chlorella sp
Software
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Summary:Microalgae is gaining popularity as a major ingredient in nutrition supplements. To mass cultivate, it is imperative to improve the biomass yield hence optimization of cultures conditions becomes paramount. In this work, an attempt has been made to optimize the microalgal production using response surface methodology (RSM) and validate further the optimized parameters. The optimum conditions for the cultivation of Chlorella sp. KPU016 under optimized nutrient conditions were pH 8.2, the light intensity of 3100 lx, glycerol 1.44 g.L-1 (under pre-set conditions of 12 h lighting, the temperature at 27±1°C. With these RSM-driven optimum conditions, the yield of microalgal biomass achieved was 282.50 mg.L-1. For larger-scale microalgal harvesting, the validated optimal conditions can be inferred as the best for enhanced microalgal production. The isolate was partially sequenced and submitted to the NCBI database and the GenBank accession number is MZ348364.
ISSN:0972-6268
2395-3454
DOI:10.46488/NEPT.2021.v20i05.028