Evaluation of Reducing Ability and Antioxidant Activity of Fruit Pomace Extracts by Spectrophotometric and Electrochemical Methods

The component profiles of apricot, grape, and black currant pomace extracts have been analyzed using HPLC coupled to diode-array detection and tandem mass spectrometry (HPLC-DAD-MS). The predominant components in grape, apricot, and black currant pomace extracts were phenolic acids and flavonols. Th...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2020, Vol.2020 (2020), p.1-16
Main Authors: Vasyliev, Georgii, Linyucheva, Olga V., Vorobyova, Victoria
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Assaying
Bioflavonoids
Calibration
Cavitation
Chromatography
Comparative analysis
Electrodes
Flavones
Flavonoids
Flavonols
Food
Fruits
Grapes
High performance liquid chromatography
Mass spectrometry
Methods
Oxidation
Phenolic acids
Potassium ferricyanide
Raw materials
Reduction
Scavenging
Scientific imaging
Spectrophotometry
Ultrasonic imaging
Vegetables
Voltammetry
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Summary:The component profiles of apricot, grape, and black currant pomace extracts have been analyzed using HPLC coupled to diode-array detection and tandem mass spectrometry (HPLC-DAD-MS). The predominant components in grape, apricot, and black currant pomace extracts were phenolic acids and flavonols. The redox behavior of apricot, black currant, and grape pomace water extracts was evaluated by means of cyclic voltammetry. Also, individual substances mainly present in the extracts were analyzed. The results of electrochemical testing were compared to traditional chemical techniques of potassium ferricyanide reduction (FRAP) and phosphomolybdenum assay, and fair agreement was established. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays were applied in order to estimate antioxidant activity. The reducing power of the grape extract was found to be higher than that of the apricot and black currant extracts in both potassium ferricyanide reduction (FRAP) and phosphomolybdenum methods.
ISSN:2090-8865
2090-8873
DOI:10.1155/2020/8869436