The Disorganization of Retinal Inner Layers Is Correlated to Müller Cells Impairment in Diabetic Macular Edema: An Imaging and Omics Study

The disorganization of retinal inner layers (DRIL) is an optical coherence tomography (OCT) biomarker strictly associated with visual outcomes in patients with diabetic macular edema (DME) whose pathophysiology is still unclear. The aim of this study was to characterize in vivo, using retinal imagin...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-06, Vol.24 (11), p.9607
Main Authors: Midena, Edoardo, Torresin, Tommaso, Schiavon, Stefano, Danieli, Luca, Polo, Chiara, Pilotto, Elisabetta, Midena, Giulia, Frizziero, Luisa
Format: Article
Language:English
Subjects:
Apoptosis
Aqueous humour
Biomarkers
Biopsy
Communication
Diabetes
Diabetes mellitus
Diabetic retinopathy
disorganization of retinal inner layers
Dropsy
Edema
Eye
Glial fibrillary acidic protein
imaging
In vivo methods and tests
inflammation
Leukocytes
macula
Medical imaging
Medical research
Medicine, Experimental
Mueller cells
Neurons
Optical Coherence Tomography
Pathogenesis
Pathophysiology
Photoreceptors
Proteins
Proteomics
Retina
Retinal images
Statistical analysis
Tomography
Type 2 diabetes
Visual acuity
Visual perception
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The disorganization of retinal inner layers (DRIL) is an optical coherence tomography (OCT) biomarker strictly associated with visual outcomes in patients with diabetic macular edema (DME) whose pathophysiology is still unclear. The aim of this study was to characterize in vivo, using retinal imaging and liquid biopsy, DRIL in eyes with DME. This was an observational cross-sectional study. Patients affected by center-involved DME were enrolled. All patients underwent spectral domain optical coherence tomography (SD-OCT) and proteomic analysis of aqueous humor (AH). The presence of DRIL at OCT was analyzed by two masked retinal experts. Fifty-seven biochemical biomarkers were analyzed from AH samples. Nineteen eyes of nineteen DME patients were enrolled. DRIL was present in 10 patients (52.63%). No statistically significant difference was found between DME eyes with and without DRIL, considering the AH concentration of all the analyzed biomarkers except for glial fibrillary acidic protein (GFAP), a biomarker of Müller cells dysfunction ( = 0.02). In conclusion, DRIL, in DME eyes, seems to strictly depend on a major dysfunction of Müller cells, explaining its role not only as imaging biomarker, but also as visual function Müller cells-related parameter.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24119607