Preconditioning with L-alanyl-glutamine reduces hepatic ischemia-reperfusion injury in rats Pré-condicionamento com L-alanil-glutamina reduz a lesão de isquemia-reperfusão no fígado de ratos

PURPOSE: To evaluate the effects of pre-conditioning with L-alanyl- glutamine (L-Ala-Gln) in rats subjected to total hepatic ischemia. METHODS: Thirty Wistar rats, average weight 300g, were randomly assigned to 3 groups (n=10): G-1 - Saline, G-2- L-Ala-Gln, G-3-control (Sham). G-1 and G-3 groups wer...

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Published in:Acta cirúrgica brasileira 2011-01, Vol.26, p.8-13
Main Authors: Raimundo José Cunha Araújo Júnior, Raimundo Gerônimo da Silva Júnior, Marcelo Pinho Pessoa de Vasconcelos, Sérgio Botelho Guimarães, Paulo Roberto Leitão de Vasconcelos, José Huygens Parente Garcia
Format: Article
Language:English
Subjects:
Fígado
Glutamina
Glutamine
Ischemia
Isquemia
Liver
Procedimentos Cirúrgicos Operatórios
Ratos
Rats
Reperfusion
Reperfusão
Surgical Procedures
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Summary:PURPOSE: To evaluate the effects of pre-conditioning with L-alanyl- glutamine (L-Ala-Gln) in rats subjected to total hepatic ischemia. METHODS: Thirty Wistar rats, average weight 300g, were randomly assigned to 3 groups (n=10): G-1 - Saline, G-2- L-Ala-Gln, G-3-control (Sham). G-1 and G-3 groups were treated with saline 2.0 ml or L-Ala-Gln (0.75mg/Kg) intraperitoneally (ip) respectively, 2 hours before laparotomy. Anesthetized rats were subjected to laparotomy and total hepatic ischemia (30 minutes) induced by by clamping of portal triad. Control group underwent peritoneal puncture, two hours before the sham operation (laparotomy only). At the end of ischemia (G1 and G2), the liver was reperfused for 60 minutes. Following reperfusion blood samples were collected for evaluation of alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels. Liver (medium lobe) was removed for immunohistochemistry study with antibody for Caspase-3. RESULTS: It was found a significant decrease (p
ISSN:0102-8650
1678-2674
DOI:10.1590/S0102-86502011000700003