MicroRNA-191 regulates oral squamous cell carcinoma cells growth by targeting PLCD1 via the Wnt/β-catenin signaling pathway

• Published in: BMC cancer 2023-07, Vol.23 (1), p.668-668, Article 668
• Main Authors: Wang, Zekun, Guan, Wenzhao, Ma, Yufeng, Zhou, Xuedong, Song, Guohua, Wei, Jianing, Wang, Chenyang
• Format: Article
• Language: English
• Subjects: Breast cancer; Cancer growth and metastasis; Cancer therapies; Cell cycle; Cell growth; Cell migration; Cell proliferation; Colorectal cancer; Enhancers; Genes; Laboratory animals; Medical prognosis; MicroRNAs; miR-191; miRNA; mRNA; Oral cancer; Oral carcinoma; Oral squamous cell carcinoma; Phospholipase C; Plasmids; PLCD1; Proteins; Radiation therapy; Reagents; Signal transduction; Squamous cell carcinoma; Surgery; Tumorigenesis; Tumors; Wnt protein; β-Catenin
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-023-11113-9

Studies have shown that microRNA-191 (miR-191) is involved in the development and progression of a variety of tumors. However, the function and mechanism of miR-191 in oral squamous cell carcinoma (OSCC) have not been clarified. The expression level of miR-191 in tumor tissues of patients with primary OSCC and OSCC cell lines were detected using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. OSCC cells were treated with miR-191 enhancers and inhibitors to investigate the effects of elevated or decreased miR-191 expression on OSCC cells proliferation, migration, cell cycle, and tumorigenesis. The target gene of miR-191 in OSCC cells were analyzed by dual-Luciferase assay, and the downstream signaling pathway of the target genes was detected using western blot assay. The expression of miR-191 was significantly upregulated in OSCC tissues and cell lines. Upregulation of miR-191 promoted proliferation, migration, invasion, and cell cycle progression of OSCC cells, as well as tumor growth in nude mice. Meanwhile, reduced expression of miR-191 inhibited these processes. Phospholipase C delta1 (PLCD1) expression was significantly downregulated, and negatively correlated with the expression of miR-191 in OSCC tissues. Dual-Luciferase assays showed that miR-191-5p could bind to PLCD1 mRNA and regulate PLCD1 protein expression. Western blot assay showed that the miR-191 regulated the expression of β-catenin and its downstream gene through targeting PLCD1. MicroRNA-191 regulates oral squamous cell carcinoma cells growth by targeting PLCD1 via the Wnt/β-catenin signaling pathway. Thus, miR-191 may serve as a potential target for the treatment of OSCC.