Phage Display Libraries for Antibody Therapeutic Discovery and Development

Phage display technology has played a key role in the remarkable progress of discovering and optimizing antibodies for diverse applications, particularly antibody-based drugs. This technology was initially developed by George Smith in the mid-1980s and applied by John McCafferty and Gregory Winter t...

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Bibliographic Details
Published in:Antibodies (Basel) 2019-08, Vol.8 (3), p.44
Main Authors: Almagro, Juan C., Pedraza-Escalona, Martha, Arrieta, Hugo Iván, Pérez-Tapia, Sonia Mayra
Format: Article
Language:English
Subjects:
affinity
Amino acids
Antibodies
Antibody libraries
antibody library design
Antigens
Binding sites
Clinical trials
Cloning
developability
Display devices
E coli
Engineering
Genes
hit rate
Libraries
naive repertoires
Panning
Peptides
Phage display
Phages
Proteins
Review
Substrates
synthetic repertoires
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Summary:Phage display technology has played a key role in the remarkable progress of discovering and optimizing antibodies for diverse applications, particularly antibody-based drugs. This technology was initially developed by George Smith in the mid-1980s and applied by John McCafferty and Gregory Winter to antibody engineering at the beginning of 1990s. Here, we compare nine phage display antibody libraries published in the last decade, which represent the state of the art in the discovery and development of therapeutic antibodies using phage display. We first discuss the quality of the libraries and the diverse types of antibody repertoires used as substrates to build the libraries, i.e., naïve, synthetic, and semisynthetic. Second, we review the performance of the libraries in terms of the number of positive clones per panning, hit rate, affinity, and developability of the selected antibodies. Finally, we highlight current opportunities and challenges pertaining to phage display platforms and related display technologies.
ISSN:2073-4468
2073-4468
DOI:10.3390/antib8030044