Competitiveness of Bradyrhizobium japonicum inoculation strain for soybean nodule occupancy

The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular i...

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Bibliographic Details
Published in:Plant, soil and environment soil and environment, 2022-01, Vol.68 (1), p.59-64
Main Authors: Miljaković, Dragana, Marinković, Jelena, Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tintor, Branislava, Đukić, Vojin
Format: Article
Language:English
Subjects:
Bradyrhizobium japonicum
Competitiveness
competitiveness for nodulation
Dry weight
glycine max
Inoculation
Nitrogen
nitrogen fixation
Nodulation
Nodules
Occupancy
Parameters
Plant growth
protein crop
rRNA 16S
Shoots
Soil conditions
Soil investigations
Soils
Soybeans
Strain analysis
symbiotic bacteria
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Summary:The competitiveness of Bradyrhizobium japonicum inoculation strain against indigenous rhizobia was examined in a soil pot experiment. The effect of inoculation strain was evaluated under different soil conditions: with or without previously grown soybean and applied commercial inoculant. Molecular identification of inoculation strain and investigated rhizobial isolates, obtained from nodules representing inoculated treatments, was performed based on 16S rDNA and enterobacterial repetitive intergenic consensus (ERIC) sequencing. Inoculation strain showed a significant effect on the investigated parameters in both soils. Higher nodule occupancy (45% vs. 18%), nodule number (111% vs. 5%), nodule dry weight (49% vs. 9%), shoot length (15% vs. 7%), root length (31% vs. 13%), shoot dry weight (34% vs. 11%), shoot nitrogen content (27% vs. 2%), and nodule nitrogen content (9% vs. 5%) was detected in soil without previously grown soybean and applied commercial inoculant. Soil had a significant effect on the shoot, root and nodule nitrogen content, while interaction of experimental factors significantly altered dry weight and nitrogen content of shoots, roots and nodules, as well as number of nodules. Nodulation parameters were significantly related with shoot dry weight, shoot and nodule nitrogen content. Symbiotic performance of inoculation strains in the field could be improved through co-selection for their competitiveness and effectiveness.
ISSN:1214-1178
1805-9368
DOI:10.17221/430/2021-PSE