Degradation Kinetics of Lignocellulolytic Enzymes in a Biogas Reactor Using Quantitative Mass Spectrometry

The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability...

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Bibliographic Details
Published in:Fermentation (Basel) 2023-01, Vol.9 (1), p.67
Main Authors: Küchler, Jan, Willenbücher, Katharina, Reiß, Elisabeth, Nuß, Lea, Conrady, Marius, Ramm, Patrice, Schimpf, Ulrike, Reichl, Udo, Szewzyk, Ulrich, Benndorf, Dirk
Format: Article
Language:English
Subjects:
Alternative energy sources
Anaerobic digestion
Biodegradability
Biogas
biogas production
By products
Cellulose
Chromatography
enzyme stability
Enzymes
fermentation
fungal enzymes
gas production (biological)
half life
Investigations
Lentinula edodes
Lignin
lignocellulases
Lignocellulose
lignocellulose conversion
Mass spectrometry
Mass spectroscopy
methane
Mushrooms
Phenols
Proteins
Reactors
Scientific imaging
Supplements
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Summary:The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance of biogas production in industrial biogas plants. Since the structural stability of these enzyme preparations is essential for efficient application, reliable methods for the assessment of enzyme stability are crucial. Here, a mass-spectrometric-based assay was established to monitor the structural stability of enzymes, i.e., the structural integrity of these proteins, in anaerobic digestion (AD). The analysis of extracts of Lentinula edodes revealed the rapid degradation of lignocellulose-degrading enzymes, with an approximate half-life of 1.5 h. The observed low structural stability of lignocellulose-degrading enzymes in AD corresponded with previous results obtained for biogas content. The established workflow can be easily adapted for the monitoring of other enzyme formulations and provides a platform for evaluating the effects of enzyme additions in AD, together with a characterization of the biochemical methane potential used in order to determine the biodegradability of organic substrates.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation9010067