Polymyxin B1 and E2 From Paenibacillus polymyxa Y-1 for Controlling Rice Bacterial Disease

• Published in: Frontiers in cellular and infection microbiology 2022-03, Vol.12, p.866357
• Main Authors: Yi, Wenshi, Chen, Chao, Gan, Xiuhai
• Format: Article
• Language: English
• Subjects: antibacterial activity; Cellular and Infection Microbiology; Paenibacillus polymyxa Y-1; phenylpropanoid biosynthesis; polymyxin; rice bacterial blight
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2022.866357

To discover novel microbial pesticide for controlling rice bacterial disease, polymyxin B 1 and E 2 were firstly isolated from the supernatant of fermentation broth of Paenibacillus polymyxa Y-1 by bioactivity tracking separation. It is shown that polymyxin B 1 and E 2 had remarkable in vitro inhibitory activities to Xanthomonas oryzae pv. oryzae ( Xoo ) and Xanthomonas oryzae pv. oryzicola ( Xoc ) with the EC 50 values of 0.19 μg/ml and 0.21 μg/ml against Xoo , and 0.32 μg/ml and 0.41 μg/ml against Xoc , respectively, which were better than those of Zhongshengmycin (0.31 μg/ml and 0.73 μg/ml) and Bismerthiazol (77.48 μg/ml and 85.30 μg/ml). Polymyxins B 1 and E 2 had good protection and curative activities against rice bacterial leaf blight (BLB) and rice bacterial leaf streak (BLS) in vivo . The protection and curative activities of polymyxins B 1 (45.8 and 35.8%, respectively) and E 2 (41.2 and 37.0%, respectively) to BLB were superior to those of Zhongshengmycin (34.8 and 29.8%, respectively) and Bismerthiazol (38.0 and 33.5%, respectively). Meanwhile, the protection and curative activities of polymyxins B 1 (44.8 and 39.8%, respectively) and E 2 (42.9 and 39.9%, respectively) to BLS were also superior to those of Zhongshengmycin (39.7 and 32.0%, respectively) and Bismerthiazol (41.5 and 34.3%, respectively). Polymyxin B 1 exerted the anti-pesticide properties via destroying the cell integrity of Xoo , reducing its infectivity and enhancing rice resistance against pathogens through activating the phenylpropanoid biosynthesis pathway of rice. It is indicated that polymyxin B 1 and E 2 were potential microbial pesticides for controlling rice bacterial disease.