Combination of Dendrobium Mixture and Metformin Curbs the Development and Progression of Diabetic Cardiomyopathy by Targeting the lncRNA NEAT1

This study aimed to explore the efficacy of combination treatment with dendrobium mixture and metformin (Met) in diabetic cardiomyopathy (DCM) and its effects on NEAT1 and the Nrf2 signaling pathway. H9c2 cells were maintained in medium supplemented with either low (5.5 mmol/L) or high (50 mmol/L) g...

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Published in:Clinics (São Paulo, Brazil) Brazil), 2021-01, Vol.76, p.e2669-e2669, Article e2669
Main Authors: Qin, Wenmin, Zhao, Xing, Tai, Jie, Qin, Guoyun, Yu, Shanshan
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Dendrobium
Dendrobium Mixture
Diabetes Mellitus
Diabetic Cardiomyopathies - drug therapy
Diabetic Cardiomyopathies - genetics
Diabetic Cardiomyopathy
Humans
Male
MEDICINE, GENERAL & INTERNAL
Metformin
MicroRNAs
miR-23a-3p
NEAT1
Nrf2
Original
Rats
Rats, Sprague-Dawley
RNA, Long Noncoding - genetics
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Summary:This study aimed to explore the efficacy of combination treatment with dendrobium mixture and metformin (Met) in diabetic cardiomyopathy (DCM) and its effects on NEAT1 and the Nrf2 signaling pathway. H9c2 cells were maintained in medium supplemented with either low (5.5 mmol/L) or high (50 mmol/L) glucose. Male Sprague-Dawley rats were fed a high-glucose diet and administered a single, low dose of streptozotocin (35 mg/kg) via intraperitoneal injection to induce the development of DM. After induction of DM, the rats were treated with dendrobium mixture (10 g/kg) and Met (0.18 g/kg) daily for 4 weeks. Next, quantitative reverse transcription (qRT)-PCR and western blotting were performed to evaluate the expression levels of target genes and proteins. Flow cytometry was performed to assess apoptosis, and hematoxylin and eosin staining was performed to evaluate the morphological changes in rat cardiac tissue. In patients with diabetes mellitus (DM) and myocardial cells and heart tissues from rats with high glucose-induced DM, NEAT1 was downregulated, and the expression levels of Nrf2 were decreased (p
ISSN:1807-5932
1980-5322
1980-5322
DOI:10.6061/clinics/2021/e2669