Human dose assessment of 68Ga-NODAGA-RGD-BBN heterodimer peptide based on animal data

Aims: Calculation of the absorbed dose in human organs is one of the first steps for developing new radiopharmaceuticals. The aim of this study is to estimate the human absorbed dose of a newly developed 68Ga-NODAGA-RGD-BBN radiolabeled compound. Materials and Methods: 68Ga-NODAGA-RGD-BBN was prepar...

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Bibliographic Details
Published in:Journal of medical physics 2022-07, Vol.47 (3), p.287-293
Main Authors: Amraee, Naeimeh, Alirezapour, Behrouz, Hosntalab, Mohammad, Yousefnia, Hassan
Format: Article
Language:English
Subjects:
68ga
Buffers
Chromatography
effective dose
High performance liquid chromatography
nodaga-rgd-bbn
Nuclear energy
Organs
Original
Peptides
Phosphates
positron emotion tomography scan
Radiation dosage
radiation dose assessment resource
Radiochemistry
Stability
Thin layer chromatography
Tumors
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Summary:Aims: Calculation of the absorbed dose in human organs is one of the first steps for developing new radiopharmaceuticals. The aim of this study is to estimate the human absorbed dose of a newly developed 68Ga-NODAGA-RGD-BBN radiolabeled compound. Materials and Methods: 68Ga-NODAGA-RGD-BBN was prepared by varying different parameters at optimized conditions. The stability of the radiolabeled peptide in phosphate-buffered saline (PBS) and in human serum was evaluated for 120 min. Afterward, the biodistribution of the complex was assessed in normal and tumor-bearing mice, at least for 120 min postinjection. Finally, the human absorbed dose of 68Ga-NODAGA-RGD-BBN was estimated based on mice data using Radiation Dose Assessment Resource and Spark method. Results: 68Ga-NODAGA-RGD-BBN was produced with radiochemical purity of more than 98% (high-performance liquid chromatography/ radio thin layer chromatography (RTLC)) with high stability in PBS buffer and in human serum at least for 2 h. The complex demonstrated high uptake in gastrin-releasing peptide receptor-expressing tumors compared to other nontarget organs. Furthermore, the dose assessment for the complex showed that the kidneys receive the highest absorbed dose in comparison with other organs. Conclusion: The result of this study showed that 68Ga-NODAGA-RGD-BBN is an effective and radiolabeled ligand for tumor detection, however more studies are still needed.
ISSN:0971-6203
1998-3913
DOI:10.4103/jmp.jmp_34_22