Immunohistochemical evaluation of myofibroblasts in odontogenic keratocyst, dentigerous cyst and different clinical variants of ameloblastoma: A comparative study

• Published in: Dental research journal 2021-01, Vol.18 (1), p.36-36
• Main Authors: Anusai, V. V., Shylaja, S., Suvarna, M., Ramanand, O. V., Reddy, E. Sharath, Vamshi, V. Raghu
• Format: Article
• Language: English
• Subjects: actins; ameloblastoma; matrix metalloproteinase 2; myofibroblasts; odontogenic cysts; Original; transforming growth factor beta1
• ISSN: 1735-3327; 2008-0255
• DOI: 10.4103/1735-3327.316653

BACKGROUNDOdontogenic cysts and tumors exhibit varying degrees of aggressiveness in their biological behavior. Odontogenic keratocyst (OKC), dentigerous cyst (DC), ameloblastoma are most common odontogenic cysts and tumors to occur in the oral cavity. Myofibroblasts (MFs) in the connective tissue stroma participate in the matrix degradation process by secreting matrix metalloproteinase 2, transforming growth factor beta1 and may contribute to variation in their biological behavior. Its activity is identified by alpha-smooth muscle actin (α-SMA) marker. With this background, the present study aims to evaluate the frequency of MFs using α-SMA to determine the biological behavior of OKC, DC, and different clinical variants of ameloblastoma. MATERIALS AND METHODSA retrospective study was carried out with total of 60 samples which include 10 cases each of OKC, DC, 30 cases of different clinical variants of ameloblastomas and 10 normal mucosa taken as controls. All are stained immunohistochemically using α-SMA and were analyzed for the same. Comparison between more than 2 groups done by one way analysis of variance test with the level of significance of P ≤ 0.0001, i.e.,