Polyvinyl Alcohol Capped Silver Nanostructures for Fortified Apoptotic Potential Against Human Laryngeal Carcinoma Cells Hep-2 Using Extremely-Low Frequency Electromagnetic Field

• Published in: International journal of nanomedicine 2024-01, Vol.19, p.9317-9332
• Main Authors: Attia, Hany G, Hamouda, Mai Abdelhalim, Alasmari, Saeed, El-Telbany, Dalia F, Alamri, Zaenah Zuhair, Qahl, Safa H, Alfaifi, Mohammad Y, Al-Sawahli, Majid Mohammad, Abd El Wahed, Sara
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents - pharmacology; apoptosis; Apoptosis - drug effects; Care and treatment; Cell Proliferation - drug effects; Cell Survival - drug effects; electromagnetic field; Electromagnetic Fields; Gene expression; Genetic aspects; Health aspects; Humans; Laryngeal cancer; laryngeal carcinoma; Laryngeal Neoplasms - drug therapy; Laryngeal Neoplasms - pathology; Metal Nanoparticles; Oxidative stress; Polyvinyl alcohol; Polyvinyl Alcohol - pharmacology; Silver - pharmacology; silver nanoparticles
• ISSN: 1178-2013; 1178-2013
• DOI: 10.2147/IJN.S453689

: Polyvinyl alcohol-capped silver nanostructures (cAgNSs) were investigated in order to enhance the cytotoxicity, pro-apoptotic, and oxidant patterns of in human laryngeal carcinoma Hep-2 cells by employing a 50 mT electromagnetic field (LEMF) for 30 min. Wet chemical reduction was used to synthesize the cAgNSs, and after they had been capped with polyvinyl alcohol, they were specifically examined for particle size analysis and structural morphology. To visualize how the silver may attach to the protein targets, a molecular docking study was conducted. Estimation of cytotoxicity, cell cycle progression supported by mRNA expression of three apoptotic-promoting genes and one apoptotic-resisting. Particle size analysis results were a mean particle size of 157.3±0.5 nm, zeta potential value of -29.6 mV±1.5 mV, and polydispersity index of 0.31±0.05. Significantly reduction of IC against Hep-2 cells by around 6-fold was concluded. Also, we obtained suppression of the proliferation of Hep-2 cells, especially in the G /G and S phases. Significant enhanced mRNA expression revealed enhanced induced CASP3, p53, and Beclin-1 mediated pro-apoptosis and induced NF-κB mediated autophagy in Hep-2 cells. Augmented levels of GR, ROS and MDA as oxidative stress biomarkers were also obtained. HE staining of Hep-2 cells exposed to cAgNSs and LEMF confirmed the enhanced apoptotic potential comparatively. By conclusion, the developed nano-sized structures with the aid of extremely-low frequency electromagnetic field were successful to fortify the anti-cancer profile of cAgNSs in Hep-2 cells.