Acid selective pro-dye for cellular compartments

A novel pro-dye approach for the acid-selective staining of the subcellular compartments for better permeability and selectivity was applied. The designed sensor has suitable physicochemical properties such as a large Stokes shift and a long-lived intracellular fluorescence. The Schiff base fragment...

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Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.15304-9, Article 15304
Main Authors: Czaplińska, Barbara, Malarz, Katarzyna, Mrozek-Wilczkiewicz, Anna, Musiol, Robert
Format: Article
Language:English
Subjects:
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639/638/403
Acids
Alzheimer's disease
Bioassays
Dyes
Endosomes
Humanities and Social Sciences
Lysosomes
Metabolism
multidisciplinary
Permeability
Physicochemical properties
Science
Science (multidisciplinary)
Solvents
Wavelengths
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Summary:A novel pro-dye approach for the acid-selective staining of the subcellular compartments for better permeability and selectivity was applied. The designed sensor has suitable physicochemical properties such as a large Stokes shift and a long-lived intracellular fluorescence. The Schiff base fragment was used for the acid-sensitive release of a fluorophore without affecting the overall stability of the biological systems. Due to the presence of an imine bond in its structure and its unique fluorescent properties, it can be presented as a “pro-dye” for acidic structures such as lysosomes. As a result of an imine bond cleavage, a new fluorescent compound is released, whose substantially shifted excitation and emission wavelengths enable a more selective and effective imaging of lysosomes and endosomes. The presented report provides the chemical, physicochemical and optical profiles as well as biological assays and theoretical calculations.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-50734-8