Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles

Currently available DNA detection techniques frequently require compromises between simplicity, speed, accuracy, and cost. Here, we propose a simple, label-free, and cost-effective DNA detection platform developed at screen-printed carbon electrodes (SPCEs) modified with reduced graphene oxide (RGO)...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors (Basel) 2023-08, Vol.13 (8), p.797
Main Authors: Chiticaru, Elena Alina, Damian, Celina Maria, Pilan, Luisa, Ioniță, Mariana
Format: Article
Language:English
Subjects:
Analysis
biosensor
Biosensors
Carbon
Care and treatment
Charge transfer
Chemical reduction
Communicable diseases
Deoxyribonucleic acid
Diagnosis
DNA
DNA detection
Electrochemical analysis
Electrochemical impedance spectroscopy
Electrochemical reactions
Electrochemistry
Electrodes
Electrolytes
Environmental monitoring
Gold
gold nanoparticles
Graphene
Health aspects
Labels
Methods
Morphology
Nanomaterials
Nanoparticles
Photoelectron spectroscopy
Photoelectrons
Potassium
Properties
Scanning electron microscopy
Single-stranded DNA
Software
Spectroscopy
Structural analysis
X ray photoelectron spectroscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Currently available DNA detection techniques frequently require compromises between simplicity, speed, accuracy, and cost. Here, we propose a simple, label-free, and cost-effective DNA detection platform developed at screen-printed carbon electrodes (SPCEs) modified with reduced graphene oxide (RGO) and gold nanoparticles (AuNPs). The preparation of the detection platform involved a two-step electrochemical procedure based on GO reduction onto SPCEs followed by the electrochemical reduction of HAuCl4 to facilitate the post-grafting reaction with AuNPs. The final sensor was fabricated by the simple physical adsorption of a single-stranded DNA (ssDNA) probe onto a AuNPs–RGO/SPCE electrode. Each preparation step was confirmed by morphological and structural characterization using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy, respectively. Furthermore, the electrochemical properties of the modified electrodes have been investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results demonstrated that the introduction of AuNPs onto RGO/SPCEs led to an enhancement in surface conductivity, a characteristic that favored an increased sensitivity in detection. The detection process relied on the change in the electrochemical signal induced by the binding of target DNA to the bioreceptor and was particularly monitored by the change in the charge transfer resistance of a [Fe(CN)6]4–/3– redox couple added in the test solution.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13080797