Photosynthetic performance and stevioside concentration are improved by the arbuscular mycorrhizal symbiosis in Stevia rebaudiana under different phosphate concentrations

• Published in: PeerJ (San Francisco, CA) CA), 2020-10, Vol.8, p.e10173-e10173, Article e10173
• Main Authors: Sarmiento-López, Luis G, López-Meyer, Melina, Sepúlveda-Jiménez, Gabriela, Cárdenas, Luis, Rodríguez-Monroy, Mario
• Format: Article
• Language: English
• Subjects: Agricultural Science; Analysis; Arbuscular mycorrhizas; Biochemistry; Biosynthesis; Biotechnology; Chlorophyll; Chlorophyll fluorescence; Colonization; Enzymes; Fungi; Glucosyltransferase; Microscopy; Morphology; Mycology; Mycorrhiza-specific phosphate transporter; Oxidases; Phosphate; Phosphate transporter; Phosphates; Phosphorus; Photosynthesis; Plant growth; Plant Science; Potassium phosphate; Rhizophagus irregularis; Roots; Stevia rebaudiana; Steviol glycosides; Stevioside; Symbiosis; Transcription; UDP-glycosyltransferases
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.10173

In plants, phosphorus (P) uptake occurs via arbuscular mycorrhizal (AM) symbiosis and through plant roots. The phosphate concentration is known to affect colonization by AM fungi, and the effect depends on the plant species. plants are valuable sources of sweetener compounds called steviol glycosides (SGs), and the principal components of SGs are stevioside and rebaudioside A. However, a detailed analysis describing the effect of the phosphate concentration on the colonization of AM fungi in the roots and the relationship of these factors to the accumulation of SGs and photochemical performance has not been performed; such an analysis was the aim of this study. The results indicated that low phosphate concentrations (20 and 200 µM KH PO ) induced a high percentage of colonization by in the roots of , while high phosphate concentrations (500 and 1,000 µM KH PO ) reduced colonization. The morphology of the colonization structure is a typical -type mycorrhiza, and a mycorrhiza-specific phosphate transporter was identified. Colonization with low phosphate concentrations improved plant growth, chlorophyll and carotenoid concentration, and photochemical performance. The transcription of the genes that encode kaurene oxidase and glucosyltransferase ( ) was upregulated in colonized plants at 200 µM KH PO , which was consistent with the observed patterns of stevioside accumulation. In contrast, at 200 µM KH PO , the transcription of and the accumulation of rebaudioside A were higher in noncolonized plants than in colonized plants. These results indicate that a low phosphate concentration improves mycorrhizal colonization and modulates the stevioside and rebaudioside A concentration by regulating the transcription of the genes that encode kaurene oxidase and glucosyltransferases, which are involved in stevioside and rebaudioside A synthesis in .