Endoplasmic reticulum stress and the unfolded protein response in skeletal muscle of subjects suffering from peritoneal sepsis

We provide a descriptive characterization of the unfolded protein response (UPR) in skeletal muscle of human patients with peritoneal sepsis and a sepsis model of C57BL/6J mice. Patients undergoing open surgery were included in a cross-sectional study and blood and skeletal muscle samples were taken...

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Bibliographic Details
Published in:Scientific reports 2022-01, Vol.12 (1), p.504-504, Article 504
Main Authors: Metzing, Uta Barbara, von Loeffelholz, Christian, Steidl, Ricardo, Romeike, Bernd, Winkler, René, Rauchfuß, Falk, Settmacher, Utz, Stoppe, Christian, Coldewey, Sina M., Weinmann, Claudia, Weickert, Martin O., Claus, Ralf A., Birkenfeld, Andreas L., Kosan, Christian, Horn, Paul
Format: Article
Language:English
Subjects:
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Aged
Animals
Antigens, CD - genetics
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - genetics
Antigens, Differentiation, Myelomonocytic - metabolism
Bcl-2 protein
Bcl-x protein
Cell death
Endoplasmic reticulum
Endoplasmic Reticulum Stress
Female
GADD34 protein
Humanities and Social Sciences
Humans
Inflammation
Inositol
Male
Mice
Mice, Inbred C57BL
Middle Aged
mRNA
multidisciplinary
Muscle, Skeletal - metabolism
Musculoskeletal system
Patients
Peritoneal Diseases - genetics
Peritoneal Diseases - metabolism
Peritoneal Diseases - physiopathology
Peritoneum
Protein folding
Protein Phosphatase 1 - genetics
Protein Phosphatase 1 - metabolism
Proteins
Science
Science (multidisciplinary)
Sepsis
Sepsis - genetics
Sepsis - metabolism
Sepsis - physiopathology
Skeletal muscle
Unfolded Protein Response
X-Box Binding Protein 1 - genetics
X-Box Binding Protein 1 - metabolism
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Summary:We provide a descriptive characterization of the unfolded protein response (UPR) in skeletal muscle of human patients with peritoneal sepsis and a sepsis model of C57BL/6J mice. Patients undergoing open surgery were included in a cross-sectional study and blood and skeletal muscle samples were taken. Key markers of the UPR and cluster of differentiation 68 (CD68) as surrogate of inflammatory injury were evaluated by real-time PCR and histochemical staining. CD68 mRNA increased with sepsis in skeletal muscle of patients and animals ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-04517-9