Oral Administration of Gongronema latifolium Leaf Extract Modulates Gut Microflora and Blood Glucose of Induced Diabetic Rats

Studies have suggested that modulation of gut microbiota is a viable therapeutic possibility for diabetes. This study evaluated the ability of an edible plant, Gongronema (G.) latifolium Benth (Asclepiadaceae), to modulate the gut microbiome and reduce blood glucose of alloxan-induced diabetic rats....

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Published in:Journal of pure & applied microbiology : an international research journal of microbiology 2021-03, Vol.15 (1), p.346-355
Main Authors: Chukwudozie, Ikechukwu K., Agbo, Martina C., Ugwu, Kenneth O., Ezeonu, Ifeoma M.
Format: Article
Language:English
Subjects:
Biological products
Blood sugar
Body weight
diabetes
dysbiosis
Glibenclamide
Glucose metabolism
gongronema latifolium
gut flora
Health aspects
hyperglycaemia
Medical research
Medicine, Botanic
Medicine, Experimental
Medicine, Herbal
microbiome
Microbiota (Symbiotic organisms)
Type 2 diabetes
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Summary:Studies have suggested that modulation of gut microbiota is a viable therapeutic possibility for diabetes. This study evaluated the ability of an edible plant, Gongronema (G.) latifolium Benth (Asclepiadaceae), to modulate the gut microbiome and reduce blood glucose of alloxan-induced diabetic rats. Thirty (30) young, male, albino rats were divided into 6 groups of 5 rats each: Group 1 comprised normal rats; Groups 2 to 4, diabetic rats treated with 200, 400 and 800 mg/Kg body weight of hydro-alcoholic leaf extract, respectively; Group 5, diabetic rats treated with 0.2 mg/Kg glibenclamide (an anti-diabetic drug); and Group 6 comprised untreated diabetic rats. Following induction of diabetes with alloxan injections, the treatments were administered twice daily on a 12-hourly basis by orogastric intubation for 21 days. Thereafter, faecal samples were collected from the animals and subjected to metagenomic analysis, to ascertain the composition and relative abundance of the gut microbiota. There were five dominant bacterial phyla in the rat gut: Firmicutes, Bacteroidetes, Actinobacteria, Spirochaetea and Proteobacteria. Induction of diabetes resulted in observable dysbiosis in the rats. However, treatment of the diabetic rats with G. latifolium extract, ameliorated the state of dysbiosis and resulted in significant increase in species like Lactobacillus (L.) johnsonii, L. reuteri and Prevotella corpri, which are associated with improved glucose metabolism. The plant extract produced the best result at the dose of 400 mg/Kg. The results from this study show that G. latifolium may be used as a therapeutic option for restoration of the microbiome in diabetic patients.
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.15.1.29