The Impact of Lighting Treatments on the Biosynthesis of Phenolic Acids in Black Wheat Seedlings

• Published in: Foods 2024-08, Vol.13 (16), p.2499
• Main Authors: Lan, Hongjie, Wang, Chunping, Yang, Zhengfei, Zhu, Jiangyu, Fang, Weiming, Yin, Yongqi
• Format: Article
• Language: English
• Subjects: Aldolase; Ammonia; Anthocyanins; Antioxidants; Barley; Biosynthesis; black wheat; Chemical properties; Chemical research; Chlorophyll; Cinnamate 4-hydroxylase; Enzymes; Ethanol; Flavonoids; Fructose-1,6-bisphosphate aldolase; Gene expression; germination; Malic enzyme; Metabolites; phenolic acid; Phenolic acids; Phenols; Phenylalanine; Phenylalanine ammonia-lyase; Photosynthesis; Physiological aspects; Plant metabolism; red light; Secondary metabolites; Seedlings; Seeds; ultraviolet; Ultraviolet radiation; Wheat
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods13162499

Light, as a crucial environmental determinant, profoundly influences the synthesis of secondary metabolites in plant metabolism. This study investigated the impacts of the red light combined with ultraviolet-A (UV-A) and ultraviolet-B (UV-B) treatments on phenolic acid biosynthesis in black wheat seedlings. The results demonstrate that the red light combined with UV-A and UV-B treatments significantly enhanced the levels of phenolic acids in black wheat seedlings, at 220.4 μg/seedling and 241.5 μg/seedling, respectively. The content of bound phenolic acids in black wheat seedlings increased by 36.0% under the UV-B treatment. The application of the UV-A/UV-B treatments markedly enhanced the activities of phenylalanine ammonia-lyase, 4-coumarate CoA ligase, and cinnamate 4-hydroxylase in black wheat seedlings while also promoting the expression levels of genes related to phenolic acid synthesis. The expression levels of and related to photosynthesis were significantly upregulated. This resulted in an augmentation in the chlorophyll content, thereby enhancing photosynthesis in black wheat seedlings. Nevertheless, the UV-A and UV-B treatments also had a significant constraining effect on the growth and development of black wheat seedlings. In addition, the UV-A and UV-B treatments increased the activity and gene expression levels of antioxidant enzymes while significantly increasing the contents of total flavonoids and anthocyanins, activating the antioxidant system. The findings reveal that light-source radiation serves as an effective method for promoting the biosynthesis of phenolic acids in black wheat seedlings.