Response of stomatal density and size in Betula ermanii to contrasting climate conditions: The contributions of genetic and environmental factors

• Published in: Ecology and evolution 2024-06, Vol.14 (6), p.e11349-n/a
• Main Authors: Cai, Yihan, Aihara, Takaki, Araki, Kyoko, Sarmah, Ragini, Tsumura, Yoshihiko, Hirota, Mitsuru
• Format: Article
• Language: English
• Subjects: Adaptability; Adaptation; Altitude; Betula ermanii; Changing environments; Climate change; Climate prediction; Climatic conditions; common garden experiment; Density; Ecophysiology; Environmental changes; Environmental factors; Genetic diversity; Genetic factors; Geographical distribution; Global warming; Harsh environments; intraspecific variation; Photosynthesis; Plastic properties; Plasticity; Population genetics; Populations; Precipitation; Seeds; Stomata; stomatal density; stomatal size; Transpiration
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.11349

As plant distribution and performance are determined by both environmental and genetic factors, clarifying the contribution of these two factors is a key for understanding plant adaptation and predicting their distribution under ongoing global warming. Betula ermanii is an ideal species for such research because of its wide distribution across diverse environments. Stomatal density and size are crucial traits that plants undergo changes in to adapt to different environments as these traits directly influence plant photosynthesis and transpiration. In this study, we conducted a multi‐location common garden experiment using B. ermanii to (1) clarify the contribution of both environmental and genetic factors to the variation in stomatal density and size of B. ermanii, (2) demonstrate the differences in the plasticity of stomatal density and size among B. ermanii populations, and (3) understand how stomatal density and size of B. ermanii would respond to increased temperature and changing precipitation patterns. Genetic factors played a more significant role in stomatal size than environmental factors, suggesting that B. ermanii struggles to adjust its stomatal size in response to a changing environment. Our results also revealed a positive correlation between stomatal size plasticity and original habitat suitability, indicating that in B. ermanii populations in harsh environments exhibit lower adaptability to environmental shifts. Although stomatal density and size of B. ermanii showed the significant responses to increased temperature and shifting precipitation patterns, the response ranges of stomatal density and size to the environmental factors varied among populations. Our findings highlighted the interplay between genetic and environmental factors in determining the intraspecific variation in stomatal density and size in B. ermanii. This indicated that certain populations of B. ermanii exhibit limited stomatal plasticity and adaptability, which could directly affect photosynthesis and transpiration, suggesting potential population‐specific fitness implications for B. ermanii under future climate change. A multi‐location common garden experiment revealed that stomata density was equally determined by both genetic and environmental factors, while genetic factors played a more significant role in stomatal size than environmental factors. Additionally, it became clear that the plasticity of stomatal size was lower in populations from relatively harsh enviro