Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates

IntroductionThe lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diag...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2023-01, Vol.18 (11), p.e0294646
Main Authors: Ajaya Basnet, Basanta Tamang, Mahendra Raj Shrestha, Lok Bahadur Shrestha, Junu Richhinbung Rai, Rajendra Maharjan, Sushila Dahal, Pradip Shrestha, Shiba Kumar Rai
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 11
container_start_page e0294646
container_title PloS one
container_volume 18
creator Ajaya Basnet
Basanta Tamang
Mahendra Raj Shrestha
Lok Bahadur Shrestha
Junu Richhinbung Rai
Rajendra Maharjan
Sushila Dahal
Pradip Shrestha
Shiba Kumar Rai
description IntroductionThe lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates.MethodsIn this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p
doi_str_mv 10.1371/journal.pone.0294646
format article
fullrecord <record><control><sourceid>doaj</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_8d379ccb97824f60a349b089e90360f9</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_8d379ccb97824f60a349b089e90360f9</doaj_id><sourcerecordid>oai_doaj_org_article_8d379ccb97824f60a349b089e90360f9</sourcerecordid><originalsourceid>FETCH-doaj_primary_oai_doaj_org_article_8d379ccb97824f60a349b089e90360f93</originalsourceid><addsrcrecordid>eNqtjEtOxDAQRC0kJIbPDVj4AjM4cXDiJUIg2LOPOk6b6chxR7ZBmlNwZZIRR2BV0qtXJcR9pQ6VbquHib9ShHBYOOJB1bYxjbkQu8rqem9qpa_Edc6TUo-6M2Ynfp5yxpxnjEWyl35dS4rym0piuRwxcjkt5ORA7CnMcsSCrhBHOWM58pg3O2GAMyssIRaaySUeCMLaZMoFosPNA9ywky5QJLfWA7iCaRMp8_qB-VZceggZ7_7yRry_vnw8v-1HhqlfEs2QTj0D9WfA6bOHVMgF7LtRt9a5wbZd3XijQDd2UJ1Fq7RR3ur__PoFau54lw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Ajaya Basnet ; Basanta Tamang ; Mahendra Raj Shrestha ; Lok Bahadur Shrestha ; Junu Richhinbung Rai ; Rajendra Maharjan ; Sushila Dahal ; Pradip Shrestha ; Shiba Kumar Rai</creator><creatorcontrib>Ajaya Basnet ; Basanta Tamang ; Mahendra Raj Shrestha ; Lok Bahadur Shrestha ; Junu Richhinbung Rai ; Rajendra Maharjan ; Sushila Dahal ; Pradip Shrestha ; Shiba Kumar Rai</creatorcontrib><description>IntroductionThe lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates.MethodsIn this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p&lt;0.05.ResultAmong the total isolates (n = 226), TCPM detected 140 (61.95%) biofilm producers, with CoNS (9/9) (p&lt;0.001) as the predominant biofilm former. When compared to TCPM, TM (n = 119) (p&lt;0.001) showed 90.8% sensitivity and 70.1% specificity, CRA (n = 88) (p = 0.123) showed 68.2% sensitivity and 42% specificity, and MCRA (n = 86) (p = 0.442) showed 65.1% sensitivity and 40% specificity. Juxtaposed to CRA, colonies formed on MCRA developed more intense black pigmentation from 24 to 96 hours. There were 77 multi-drug-resistant (MDR)-biofilm formers and 39 extensively drug-resistant (XDR)-biofilm formers, with 100% resistance to ampicillin and ceftazidime, respectively.ConclusionIt is suggested that TM be used for biofilm detection, after TCPM. Unlike MCRA, black pigmentation in colonies formed on CRA declined with time. MDR- and XDR-biofilm formers were frequent among the clinical isolates.</description><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0294646</identifier><language>eng</language><publisher>Public Library of Science (PLoS)</publisher><ispartof>PloS one, 2023-01, Vol.18 (11), p.e0294646</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ajaya Basnet</creatorcontrib><creatorcontrib>Basanta Tamang</creatorcontrib><creatorcontrib>Mahendra Raj Shrestha</creatorcontrib><creatorcontrib>Lok Bahadur Shrestha</creatorcontrib><creatorcontrib>Junu Richhinbung Rai</creatorcontrib><creatorcontrib>Rajendra Maharjan</creatorcontrib><creatorcontrib>Sushila Dahal</creatorcontrib><creatorcontrib>Pradip Shrestha</creatorcontrib><creatorcontrib>Shiba Kumar Rai</creatorcontrib><title>Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates</title><title>PloS one</title><description>IntroductionThe lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates.MethodsIn this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p&lt;0.05.ResultAmong the total isolates (n = 226), TCPM detected 140 (61.95%) biofilm producers, with CoNS (9/9) (p&lt;0.001) as the predominant biofilm former. When compared to TCPM, TM (n = 119) (p&lt;0.001) showed 90.8% sensitivity and 70.1% specificity, CRA (n = 88) (p = 0.123) showed 68.2% sensitivity and 42% specificity, and MCRA (n = 86) (p = 0.442) showed 65.1% sensitivity and 40% specificity. Juxtaposed to CRA, colonies formed on MCRA developed more intense black pigmentation from 24 to 96 hours. There were 77 multi-drug-resistant (MDR)-biofilm formers and 39 extensively drug-resistant (XDR)-biofilm formers, with 100% resistance to ampicillin and ceftazidime, respectively.ConclusionIt is suggested that TM be used for biofilm detection, after TCPM. Unlike MCRA, black pigmentation in colonies formed on CRA declined with time. MDR- and XDR-biofilm formers were frequent among the clinical isolates.</description><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqtjEtOxDAQRC0kJIbPDVj4AjM4cXDiJUIg2LOPOk6b6chxR7ZBmlNwZZIRR2BV0qtXJcR9pQ6VbquHib9ShHBYOOJB1bYxjbkQu8rqem9qpa_Edc6TUo-6M2Ynfp5yxpxnjEWyl35dS4rym0piuRwxcjkt5ORA7CnMcsSCrhBHOWM58pg3O2GAMyssIRaaySUeCMLaZMoFosPNA9ywky5QJLfWA7iCaRMp8_qB-VZceggZ7_7yRry_vnw8v-1HhqlfEs2QTj0D9WfA6bOHVMgF7LtRt9a5wbZd3XijQDd2UJ1Fq7RR3ur__PoFau54lw</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Ajaya Basnet</creator><creator>Basanta Tamang</creator><creator>Mahendra Raj Shrestha</creator><creator>Lok Bahadur Shrestha</creator><creator>Junu Richhinbung Rai</creator><creator>Rajendra Maharjan</creator><creator>Sushila Dahal</creator><creator>Pradip Shrestha</creator><creator>Shiba Kumar Rai</creator><general>Public Library of Science (PLoS)</general><scope>DOA</scope></search><sort><creationdate>20230101</creationdate><title>Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates</title><author>Ajaya Basnet ; Basanta Tamang ; Mahendra Raj Shrestha ; Lok Bahadur Shrestha ; Junu Richhinbung Rai ; Rajendra Maharjan ; Sushila Dahal ; Pradip Shrestha ; Shiba Kumar Rai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-doaj_primary_oai_doaj_org_article_8d379ccb97824f60a349b089e90360f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ajaya Basnet</creatorcontrib><creatorcontrib>Basanta Tamang</creatorcontrib><creatorcontrib>Mahendra Raj Shrestha</creatorcontrib><creatorcontrib>Lok Bahadur Shrestha</creatorcontrib><creatorcontrib>Junu Richhinbung Rai</creatorcontrib><creatorcontrib>Rajendra Maharjan</creatorcontrib><creatorcontrib>Sushila Dahal</creatorcontrib><creatorcontrib>Pradip Shrestha</creatorcontrib><creatorcontrib>Shiba Kumar Rai</creatorcontrib><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ajaya Basnet</au><au>Basanta Tamang</au><au>Mahendra Raj Shrestha</au><au>Lok Bahadur Shrestha</au><au>Junu Richhinbung Rai</au><au>Rajendra Maharjan</au><au>Sushila Dahal</au><au>Pradip Shrestha</au><au>Shiba Kumar Rai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates</atitle><jtitle>PloS one</jtitle><date>2023-01-01</date><risdate>2023</risdate><volume>18</volume><issue>11</issue><spage>e0294646</spage><pages>e0294646-</pages><eissn>1932-6203</eissn><abstract>IntroductionThe lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates.MethodsIn this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p&lt;0.05.ResultAmong the total isolates (n = 226), TCPM detected 140 (61.95%) biofilm producers, with CoNS (9/9) (p&lt;0.001) as the predominant biofilm former. When compared to TCPM, TM (n = 119) (p&lt;0.001) showed 90.8% sensitivity and 70.1% specificity, CRA (n = 88) (p = 0.123) showed 68.2% sensitivity and 42% specificity, and MCRA (n = 86) (p = 0.442) showed 65.1% sensitivity and 40% specificity. Juxtaposed to CRA, colonies formed on MCRA developed more intense black pigmentation from 24 to 96 hours. There were 77 multi-drug-resistant (MDR)-biofilm formers and 39 extensively drug-resistant (XDR)-biofilm formers, with 100% resistance to ampicillin and ceftazidime, respectively.ConclusionIt is suggested that TM be used for biofilm detection, after TCPM. Unlike MCRA, black pigmentation in colonies formed on CRA declined with time. MDR- and XDR-biofilm formers were frequent among the clinical isolates.</abstract><pub>Public Library of Science (PLoS)</pub><doi>10.1371/journal.pone.0294646</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier EISSN: 1932-6203
ispartof PloS one, 2023-01, Vol.18 (11), p.e0294646
issn 1932-6203
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_8d379ccb97824f60a349b089e90360f9
source Publicly Available Content Database; PubMed Central
title Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T08%3A51%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-doaj&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20four%20in%20vitro%20phenotypic%20biofilm%20detection%20methods%20in%20relation%20to%20antimicrobial%20resistance%20in%20aerobic%20clinical%20bacterial%20isolates&rft.jtitle=PloS%20one&rft.au=Ajaya%20Basnet&rft.date=2023-01-01&rft.volume=18&rft.issue=11&rft.spage=e0294646&rft.pages=e0294646-&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0294646&rft_dat=%3Cdoaj%3Eoai_doaj_org_article_8d379ccb97824f60a349b089e90360f9%3C/doaj%3E%3Cgrp_id%3Ecdi_FETCH-doaj_primary_oai_doaj_org_article_8d379ccb97824f60a349b089e90360f93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true