Importance of Microbiome of Fecal Samples Obtained from Adolescents with Different Weight Conditions on Resistance Gene Transfer

• Published in: Microorganisms (Basel) 2022-10, Vol.10 (10), p.1995
• Main Authors: Navarro, Armando, Rodea, Gerardo E, Castelán-Sánchez, Hugo G, Saucedo-Pastrana, Héctor Armando, Licona-Moreno, Delia, Eslava-Campos, Carlos, Tirado-Gómez, Laura L, Vilchis-Reyes, Ariel, García de la Torre, Guadalupe, Cruz-Licea, Verónica
• Format: Article
• Language: English
• Subjects: Adolescents; Aminoglycosides; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bacteria; Body mass index; Body weight; Demographic aspects; Drug resistance; Drug resistance in microorganisms; E coli; Eating behavior; Feces; Fluoroquinolones; Gene transfer; Genes; gut microbiota; Health aspects; Horizontal transfer; Laboratories; Lincosamides; Medical research; Medicine, Experimental; Microbiological research; microbiome; Microbiomes; Microbiota; Microorganisms; Pathogens; Public health; Questionnaires; resistance genes; Streptococcus infections; Sulfonamides; Teenagers; Tetracyclines; Urine; Youth; β Lactamase; β-Lactam antibiotics
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10101995

Antimicrobial resistance (AMR) is a relevant public health problem worldwide, and microbiome bacteria may contribute to the horizontal gene transfer associated with antimicrobial resistance. The microbiome of fecal samples from Mexican adolescents were analyzed and correlated with eating habits, and the presence of AMR genes on bacteria in the microbiome was evaluated. Fecal samples from adolescents were collected and processed to extract genomic DNA. An Illumina HiSeq 1500 system was used to determine resistance genes and the microbiome of adolescents through the amplification of gene resistance and the V3–V4 regions of RNA, respectively. Analysis of the microbiome from fecal samples taken from 18 obese, overweight, and normal-weight adolescents revealed that the Firmicutes was the most frequent phylum, followed by Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The following species were detected as the most frequent in the samples: F. prausnitzii, P. cori, B. adolescentis, E. coli and A. muciniphila. The presence of Bacteroides, Prevotella and Ruminococcus was used to establish the enterotype; enterotype 1 was more common in women and enterotype 2 was more common in men. Twenty-nine AMR genes were found for β-lactamases, fluoroquinolones, aminoglycosides, macrolide, lincosamides, streptogramin (MLS), tetracyclines and sulfonamides. The presence of microorganisms in fecal samples that harbor AMR genes that work against antimicrobials frequently used for the treatment of microbial infections such as b-lactams, macrolides, aminoglycosides, MLS, and tetracyclines is of great concern, as these organisms may be an important reservoir for horizontal AMR gene transfer.