Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland

Despite numerous reports of Anaplasmataceae agents in mammals worldwide, few studies have investigated their occurrence in birds. The present study aimed to investigate the occurrence and molecular identity of Anaplasmataceae agents in birds from the Pantanal wetland, Brazil. Blood samples were coll...

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Bibliographic Details
Published in:Microorganisms (Basel) 2024-05, Vol.12 (5), p.962
Main Authors: Alabí Córdova, Amir Salvador, Fecchio, Alan, Calchi, Ana Cláudia, Dias, Clara Morato, Mongruel, Anna Claudia Baumel, das Neves, Lorena Freitas, Lee, Daniel Antonio Braga, Machado, Rosangela Zacarias, André, Marcos Rogério
Format: Article
Language:English
Subjects:
Actin
Anaplasma
Anaplasma phagocytophilum
Anaplasma spp
Anaplasmataceae
Arachnids
avian hosts
Basileuterus flaveolus
Bird migration
Birds
blood
Brazil
Candidatus Allocrytoplasma spp
Crax
DNA
Ehrlichia
Ehrlichia spp
Elaenia albiceps
Genes
Genetic aspects
Genetic testing
Genotypes
Gram-negative bacteria
GroEL gene
Health aspects
Owls
Pantanal
Pathogens
Polymerase chain reaction
Reagents
rRNA 16S
South America
species
Spleen
Taxonomy
Tick-borne diseases
Vectors (Biology)
Wetlands
Zoonoses
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Summary:Despite numerous reports of Anaplasmataceae agents in mammals worldwide, few studies have investigated their occurrence in birds. The present study aimed to investigate the occurrence and molecular identity of Anaplasmataceae agents in birds from the Pantanal wetland, Brazil. Blood samples were collected from 93 different species. After DNA extraction, samples positive for the avian β-actin gene were subjected to both a multiplex quantitative real-time (q)PCR for and targeting the gene and to a conventional PCR for Anaplasmataceae agents targeting the 16S rRNA gene. As a result, 37 (7.4%) birds were positive for spp. and 4 (0.8%) for spp. in the qPCR assay; additionally, 13 (2.6%) were positive for Anaplasmataceae agents in the PCR targeting the 16S rRNA gene. The 16S rRNA sequences detected in , , and were positioned closely to sp. Magellanica. sequences detected in and grouped with . The 16S rRNA genotypes detected in and grouped with Allocryptoplasma. The 23S-5S genotypes detected in , , and were related to . In conclusion, novel genotypes of , , and Allocryptoplasma were detected in birds from the Pantanal wetland.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12050962