Fibrogenesis and epithelial coating of skin wounds in rats treated with angico extract (Anadenanthera colubrina var. cebil)

• Published in: Acta cirúrgica brasileira 2015-05, Vol.30 (5), p.353-358
• Main Authors: Pessoa, Wagner Soares, Estevão, Lígia Reis de Moura, Simões, Ricardo Santos, Mendonça, Fábio de Souza, Evêncio-Luz, Luis, Baratella-Evêncio, Liriane, Florencio-Silva, Rinaldo, Sá, Fabrício Bezerra de, Evêncio-Neto, Joaquim
• Format: Article
• Language: English
• Subjects: Collagen; Fibroblasts; Phytotherapy; Rats; Wound Healing
• ISSN: 0102-8650; 0102-8650
• DOI: 10.1590/S0102-865020150050000007

PURPOSE: To evaluate the effects of angico bark extract (Anadenanthera colubrina var. cebil) in the healing process of the skin of rats.METHODS: Twenty adult male rats were divided into four groups of five animals each, according to the respective postoperative days, as follow: G4, G7, G14 and G21. Each group received two incisions on skin and subcutaneous tissue in the right and left antimere of the thoracic region, separated by a distance of 2 cm. The right lesion was treated daily with saline and the left with the angico alcoholic extract (5%). At the end of each experimental period, the animals were euthanized and fragments of the wound area with the edges were removed, fixed in 10% formaldehyde solution and processed for paraffin embedding. Histological sections (5 μm of thickness) were stained with hematoxylin and eosin (HE), Gomori trichromic and picrosisirus red for morphological and morphometric analyses. Statistical analysis was done by ANOVA and Tukey-Kramer test (p