Antiradical activity of Paulownia tomentosa (Scrophulariaceae) extracts

• Published in: Molecules (Basel, Switzerland) Switzerland), 2007-06, Vol.12 (6), p.1210-1219
• Main Authors: Smejkal, Karel, Holubova, Pavla, Zima, Ales, Muselik, Jan, Dvorska, Margita
• Format: Article
• Language: English
• Subjects: antioxidant; antiperoxynitrite; DPPH; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Flavonoids; Flavonoids - isolation & purification; Flavonoids - pharmacology; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; Free radicals; Fruit - chemistry; Paulownia tomentosa; Pharmaceutical sciences; Pharmacy; phenolics; Phenols - isolation & purification; Phenols - pharmacology; Plants, Medicinal; Polyphenols; Solvents; Trees
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/12061210

Paulownia tomentosa is a large indecidous tree planted mostly for its fast growing wood and decorative purposes. The tree is also used in traditional Chinese medicine. As a part of our study of natural polyphenols, the fruits of Paulownia tomentosa were extracted by EtOH and than subjected to liquid/liquid extraction. Fractions were analysed by TLC and HPLC to determine presence of phenolic substances. We identified and quantified acteoside (1) and isoacteoside (2) in the EtOAc and n-BuOH extracts; mimulone (3) and diplacone (4) in the MeOH extract. To determine the antiradical activity of extracts we used the anti DPPH and peroxynitrite assays. The activity was expressed as Trolox C equivalents, IC50 for DPPH scavenging and a time dependency course was established. The polyphenols content was determined; results were expressed as gallic acid equivalents. Using these methods we found the fractions of the n-BuOH, EtOAc and MeOH extracts that display antiradical activity, which could be exploited as potential pharmaceuticals.