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The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose

The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative...

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Published in:Brazilian archives of biology and technology 2006-07, Vol.49 (4), p.683-687
Main Authors: Vignoli, Josiane Alessandra, Celligoi, Maria Antonia Colabone, Silva, Rui Sérgio Ferreira da, Barros, Márcio de
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container_title Brazilian archives of biology and technology
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Celligoi, Maria Antonia Colabone
Silva, Rui Sérgio Ferreira da
Barros, Márcio de
description The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol. Foi investigado a produção de sorbitol em meio de sacarose por células de Z. mobilis permeabilizadas e imobilizadas em Luffa cylindrica. Este trabalho avaliou o efeito da permeabilização de células de Z. mobilis tratadas com cetilmetilamoniobrometo e imobilizadas em Luffa cylindrinca. Um planejamento fatorial completo 2³ foi utilizado para verificar a influência dos fatores e suas interações. A permeabilização da célula mostrou um efeito significante e negativo sobre a produção de sorbitol, enquanto o tempo de cultivo e o processo de imobilização foram significantes e positivos. Os resultados mostraram que a imobilização das células e o tempo de cultivo de 36h forneceram concentração mais elevadas de sorbitol.
doi_str_mv 10.1590/S1516-89132006000500020
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identifier ISSN: 1516-8913
ispartof Brazilian archives of biology and technology, 2006-07, Vol.49 (4), p.683-687
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1516-8913
1678-4324
language eng
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source IngentaConnect Journals; SciELO
subjects immobilization
Luffa cylindrica
Permeabilization
sucrose
Z. mobilis
Zymomonas mobilis
title The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose
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