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The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose
The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative...
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Published in: | Brazilian archives of biology and technology 2006-07, Vol.49 (4), p.683-687 |
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container_title | Brazilian archives of biology and technology |
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creator | Vignoli, Josiane Alessandra Celligoi, Maria Antonia Colabone Silva, Rui Sérgio Ferreira da Barros, Márcio de |
description | The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol.
Foi investigado a produção de sorbitol em meio de sacarose por células de Z. mobilis permeabilizadas e imobilizadas em Luffa cylindrica. Este trabalho avaliou o efeito da permeabilização de células de Z. mobilis tratadas com cetilmetilamoniobrometo e imobilizadas em Luffa cylindrinca. Um planejamento fatorial completo 2³ foi utilizado para verificar a influência dos fatores e suas interações. A permeabilização da célula mostrou um efeito significante e negativo sobre a produção de sorbitol, enquanto o tempo de cultivo e o processo de imobilização foram significantes e positivos. Os resultados mostraram que a imobilização das células e o tempo de cultivo de 36h forneceram concentração mais elevadas de sorbitol. |
doi_str_mv | 10.1590/S1516-89132006000500020 |
format | article |
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Foi investigado a produção de sorbitol em meio de sacarose por células de Z. mobilis permeabilizadas e imobilizadas em Luffa cylindrica. Este trabalho avaliou o efeito da permeabilização de células de Z. mobilis tratadas com cetilmetilamoniobrometo e imobilizadas em Luffa cylindrinca. Um planejamento fatorial completo 2³ foi utilizado para verificar a influência dos fatores e suas interações. A permeabilização da célula mostrou um efeito significante e negativo sobre a produção de sorbitol, enquanto o tempo de cultivo e o processo de imobilização foram significantes e positivos. Os resultados mostraram que a imobilização das células e o tempo de cultivo de 36h forneceram concentração mais elevadas de sorbitol.</description><identifier>ISSN: 1516-8913</identifier><identifier>EISSN: 1516-8913</identifier><identifier>EISSN: 1678-4324</identifier><identifier>DOI: 10.1590/S1516-89132006000500020</identifier><language>eng</language><publisher>Instituto de Tecnologia do Paraná (Tecpar)</publisher><subject>immobilization ; Luffa cylindrica ; Permeabilization ; sucrose ; Z. mobilis ; Zymomonas mobilis</subject><ispartof>Brazilian archives of biology and technology, 2006-07, Vol.49 (4), p.683-687</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-8b5c5e43d2297e94a8c818de82b4d509490965b31295598832749f9a8e9728283</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Vignoli, Josiane Alessandra</creatorcontrib><creatorcontrib>Celligoi, Maria Antonia Colabone</creatorcontrib><creatorcontrib>Silva, Rui Sérgio Ferreira da</creatorcontrib><creatorcontrib>Barros, Márcio de</creatorcontrib><title>The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose</title><title>Brazilian archives of biology and technology</title><description>The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol.
Foi investigado a produção de sorbitol em meio de sacarose por células de Z. mobilis permeabilizadas e imobilizadas em Luffa cylindrica. Este trabalho avaliou o efeito da permeabilização de células de Z. mobilis tratadas com cetilmetilamoniobrometo e imobilizadas em Luffa cylindrinca. Um planejamento fatorial completo 2³ foi utilizado para verificar a influência dos fatores e suas interações. A permeabilização da célula mostrou um efeito significante e negativo sobre a produção de sorbitol, enquanto o tempo de cultivo e o processo de imobilização foram significantes e positivos. Os resultados mostraram que a imobilização das células e o tempo de cultivo de 36h forneceram concentração mais elevadas de sorbitol.</description><subject>immobilization</subject><subject>Luffa cylindrica</subject><subject>Permeabilization</subject><subject>sucrose</subject><subject>Z. mobilis</subject><subject>Zymomonas mobilis</subject><issn>1516-8913</issn><issn>1516-8913</issn><issn>1678-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNplUT1PwzAQjRBIlMJvwBNb4Xy2E3tEFR-VKjFQli6Wk1zBVVIXOx3Kr6dpoUJiON33O717WXbN4ZYrA3evXPF8pA0XCJADgNoZwkk2ODZO_8Tn2UVKSwAucy4H2Xz2QWwdQ72pOh9WLCxYCrH0XWhYuWVrii250jf-i2rmVjXzbRt-84qaJvUr81t2qCbmVyxtqhgSXWZnC9ckuvrxw-zt8WE2fh5NX54m4_vpqJISu5EuVaVIihrRFGSk05XmuiaNpawVGGnA5KoUHI1SRmuBhTQL4zSZAjVqMcwmB9w6uKVdR9-6uLXBebsvhPhuXex81ZDVtQHMkXLkuXSKDAghVAECqIfrsW4OWLuXfG4odbb1qafpVhQ2ySJIFGhwN1gcBnuqKdLieJiD7XWxe13sP13EN9qxfdM</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Vignoli, Josiane Alessandra</creator><creator>Celligoi, Maria Antonia Colabone</creator><creator>Silva, Rui Sérgio Ferreira da</creator><creator>Barros, Márcio de</creator><general>Instituto de Tecnologia do Paraná (Tecpar)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>DOA</scope></search><sort><creationdate>20060701</creationdate><title>The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose</title><author>Vignoli, Josiane Alessandra ; Celligoi, Maria Antonia Colabone ; Silva, Rui Sérgio Ferreira da ; Barros, Márcio de</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-8b5c5e43d2297e94a8c818de82b4d509490965b31295598832749f9a8e9728283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>immobilization</topic><topic>Luffa cylindrica</topic><topic>Permeabilization</topic><topic>sucrose</topic><topic>Z. mobilis</topic><topic>Zymomonas mobilis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vignoli, Josiane Alessandra</creatorcontrib><creatorcontrib>Celligoi, Maria Antonia Colabone</creatorcontrib><creatorcontrib>Silva, Rui Sérgio Ferreira da</creatorcontrib><creatorcontrib>Barros, Márcio de</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Directory of Open Access Journals</collection><jtitle>Brazilian archives of biology and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vignoli, Josiane Alessandra</au><au>Celligoi, Maria Antonia Colabone</au><au>Silva, Rui Sérgio Ferreira da</au><au>Barros, Márcio de</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose</atitle><jtitle>Brazilian archives of biology and technology</jtitle><date>2006-07-01</date><risdate>2006</risdate><volume>49</volume><issue>4</issue><spage>683</spage><epage>687</epage><pages>683-687</pages><issn>1516-8913</issn><eissn>1516-8913</eissn><eissn>1678-4324</eissn><abstract>The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol.
Foi investigado a produção de sorbitol em meio de sacarose por células de Z. mobilis permeabilizadas e imobilizadas em Luffa cylindrica. Este trabalho avaliou o efeito da permeabilização de células de Z. mobilis tratadas com cetilmetilamoniobrometo e imobilizadas em Luffa cylindrinca. Um planejamento fatorial completo 2³ foi utilizado para verificar a influência dos fatores e suas interações. A permeabilização da célula mostrou um efeito significante e negativo sobre a produção de sorbitol, enquanto o tempo de cultivo e o processo de imobilização foram significantes e positivos. Os resultados mostraram que a imobilização das células e o tempo de cultivo de 36h forneceram concentração mais elevadas de sorbitol.</abstract><pub>Instituto de Tecnologia do Paraná (Tecpar)</pub><doi>10.1590/S1516-89132006000500020</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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source | IngentaConnect Journals; SciELO |
subjects | immobilization Luffa cylindrica Permeabilization sucrose Z. mobilis Zymomonas mobilis |
title | The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose |
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