Genome-wide association study provides new insight into the underlying mechanism of drought tolerance during seed germination stage in soybean

• Published in: Scientific reports 2024-09, Vol.14 (1), p.20765-14, Article 20765
• Main Authors: Aleem, Muqadas, Razzaq, Muhammad Khuram, Aleem, Maida, Yan, Wenliang, Sharif, Iram, Siddiqui, Manzer H., Aleem, Saba, Iftikhar, Muhammad Sarmad, Karikari, Benjamin, Ali, Zulfiqar, Begum, Naheeda, Zhao, Tuanjie
• Format: Article
• Language: English
• Subjects: 631/208; 631/449; 631/61; Chromosome 8; Crop yield; Developmental stages; Drought; Drought Resistance; Droughts; Environmental factors; Gene regulation; Genetic factors; Genome wide association study; Genome-wide association studies; Genomes; Genotype; Genotypes; Germination; Germination - genetics; Germplasm; Glycine max; Glycine max - genetics; Glycine max - growth & development; Glycine max - physiology; Heritability; Humanities and Social Sciences; multidisciplinary; Nucleotides; Phenotype; Plant breeding; Polymorphism, Single Nucleotide; QTNs; Quantitative Trait Loci; Science; Science (multidisciplinary); Seed germination; Seedlings; Seeds - genetics; Seeds - growth & development; Single-nucleotide polymorphism; SNPs; Soybean; Soybeans; Stress, Physiological - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-71357-8

Drought is one of the major environmental issues that reduce crop yield. Seed germination is a crucial stage of plant development in all crop plants, including soybean. In soybean breeding, information about genetic mechanism of drought tolerance has great importance. However, at germination stage, there is relatively little knowledge on the genetic basis of soybean drought resistance. The objective of this work was to find the quantitative trait nucleotides (QTNs) linked to drought tolerance related three traits using a genome-wide association study (GWAS), viz., germination rate (GR), root length (RL), and whole seedling length (WSL), using germplasm population of 240 soybean PIs with 34,817 SNPs genotype data having MAF > 0.05. It was observed that heritability (H 2 ) for GR, WSL, and RL across both environments (2020, and 2019) were high in the range of 0.76–0.99, showing that genetic factors play a vital role in drought tolerance as compared to environmental factors. A number of 23 and 27 QTNs were found to be linked to three traits using MLM and mrMLM, respectively. Three significant QTNs, qGR8-1, qWSL13-1, and qRL-8, were identified using both MLM and mrMLM methods among these QTNs. QTN8 , located on chromosome 8 was consistently linked to two traits (GR and RL). The area (± 100 Kb) associated with this QTN was screened for drought tolerance based on gene annotation. Fifteen candidate genes were found by this screening. Based on the expression data, four candidate genes i.e. Glyma08g156800 , Glyma08g160000 , Glyma08g162700 , and Glyma13g249600 were found to be linked to drought tolerance regulation in soybean. Hence, the current study provides evidence to understand the genetic constitution of drought tolerance during the germination stage and identified QTNs or genes could be utilized in molecular breeding to enhance the yield under drought stress.