Using Temporally and Spatially Resolved Measurements to Improve the Sensitivity of Fluorescence-Based Immunoassays

Detecting low concentrations of biomarkers is essential in clinical laboratories. To improve analytical sensitivity, especially in identifying fluorescently labeled molecules, typical optical detection systems, consisting of a photodetector or camera, utilize time-resolved measurements. Taking a dif...

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Published in:Biosensors (Basel) 2024-04, Vol.14 (5), p.220
Main Authors: Kremer, Ran, Roth, Shira, Bross, Avital, Danielli, Amos, Noam, Yair
Format: Article
Language:English
Subjects:
Aggregates
Algorithms
Antibodies
Biomarkers
Biomarkers - analysis
Biosensing Techniques
Biosensors
Cytokines
Digital cameras
Fluorescence
Fluorescent indicators
Humans
image processing
Immunoassay
Immunoassay - methods
Immunoassays
in vitro diagnostics
Interleukin 8
Interleukin-8 - analysis
Lasers
Limit of Detection
Low concentrations
magnetic beads
Magnetic fields
Methods
Quantum dots
Sensitivity analysis
Signal processing
Spectrum analysis
Target detection
Time measurement
Zika virus
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container_issue 5
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creator Kremer, Ran
Roth, Shira
Bross, Avital
Danielli, Amos
Noam, Yair
description Detecting low concentrations of biomarkers is essential in clinical laboratories. To improve analytical sensitivity, especially in identifying fluorescently labeled molecules, typical optical detection systems, consisting of a photodetector or camera, utilize time-resolved measurements. Taking a different approach, magnetic modulation biosensing (MMB) is a novel technology that combines fluorescently labeled probes and magnetic particles to create a sandwich assay with the target molecules. By concentrating the target molecules and then using time-resolved measurements, MMB provides the rapid and highly sensitive detection of various biomarkers. Here, we propose a novel signal-processing algorithm that enhances the detection and estimation of target molecules at low concentrations. By incorporating both temporally and spatially resolved measurements using human interleukin-8 as a target molecule, we show that the new algorithm provides a 2-4-fold improvement in the limit of detection and an ~25% gain in quantitative resolution.
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subjects Aggregates
Algorithms
Antibodies
Biomarkers
Biomarkers - analysis
Biosensing Techniques
Biosensors
Cytokines
Digital cameras
Fluorescence
Fluorescent indicators
Humans
image processing
Immunoassay
Immunoassay - methods
Immunoassays
in vitro diagnostics
Interleukin 8
Interleukin-8 - analysis
Lasers
Limit of Detection
Low concentrations
magnetic beads
Magnetic fields
Methods
Quantum dots
Sensitivity analysis
Signal processing
Spectrum analysis
Target detection
Time measurement
Zika virus
title Using Temporally and Spatially Resolved Measurements to Improve the Sensitivity of Fluorescence-Based Immunoassays
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