Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts

Reconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-05, Vol.12 (1), p.9073-9073, Article 9073
Main Authors: Moreau, Marielle, Capallere, Christophe, Chavatte, Laurent, Plaza, Christelle, Meyrignac, Céline, Pays, Karl, Bavouzet, Bruno, Botto, Jean-Marie, Nizard, Carine, Bulteau, Anne-Laure
Format: Article
Language:English
Subjects:
631/532/2435
631/532/2438
631/80/642/333/1465
Adenosine triphosphate
Animal research
Animals
Bioenergetics
Epidermal Cells
Epidermis
Epidermis - metabolism
Fibroblasts
Humanities and Social Sciences
Humans
Keratinocytes
Keratinocytes - metabolism
Life Sciences
Metabolism
Mitochondria
multidisciplinary
Pharmaceuticals
Phosphorylation
Plant extracts
Plant Extracts - metabolism
Plant Extracts - pharmacology
Pluripotency
Respiration
Science
Science (multidisciplinary)
Skin
Stem cell transplantation
Stem cells
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Reconstructed human epidermis equivalents (RHE) have been developed as a clinical skin substitute and as the replacement for animal testing in both research and industry. KiPS, or keratinocytes derived from induced pluripotent stem cells (iPSCs) are frequently used to generate RHE. In this study, we focus on the mitochondrial performance of the KiPS derived from iPSCs obtained from two donors. We found that the KiPS derived from the older donor have more defective mitochondria. Treatment of these KiPS with a plant extract enriched in compounds known to protect mitochondria improved mitochondrial respiration and rendered them fully competent to derive high-quality RHE. Overall, our results suggest that improving mitochondrial function in KiPS is one of the key aspects to obtain a functional RHE and that our plant extracts can improve in this process.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-13191-4