Examining the impact of zinc on horizontal gene transfer in Enterobacterales

• Published in: Scientific reports 2022-11, Vol.12 (1), p.20503-20503, Article 20503
• Main Authors: Ekhlas, Daniel, Soro, Arturo B., Leonard, Finola C., Manzanilla, Edgar G., Burgess, Catherine M.
• Format: Article
• Language: English
• Subjects: 631/326; 631/326/22; 631/326/22/1434; Ampicillin; Animals; Antimicrobial agents; Antimicrobial resistance; Conjugation; Conjugation, Genetic; Drug resistance; E coli; Escherichia coli - genetics; Gammaproteobacteria; Gene transfer; Gene Transfer, Horizontal; Growth rate; Heavy metals; Horizontal transfer; Humanities and Social Sciences; Humans; multidisciplinary; Multidrug resistance; Rifampin; Salmonella; Science; Science (multidisciplinary); Strains (organisms); Zinc; Zinc - pharmacology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-23690-z

Antimicrobial resistance is one of the main international health concerns for humans, animals, and the environment, and substantial efforts have focused on reducing its development and spread. While there is evidence for correlations between antimicrobial usage and antimicrobial resistance development, specific information on the effect of heavy metal/antimicrobial usage on bacterial conjugation is more limited. The aim of this study was to investigate the effects of zinc and antimicrobials in different concentrations on horizontal gene transfer of an ampicillin resistance gene, using a multi-drug resistant Escherichia coli donor strain and three different Salmonella enterica serovars as recipient strains. Differences in conjugation frequencies for the different Salmonella recipients were observed, independent of the presence of zinc or the antimicrobials. Selective pressure on the recipient strains, in the form of ampicillin, resulted in a decrease in conjugation frequencies, while, the presence of rifampicin resulted in increases. Zinc exposure affected conjugation frequencies of only one of the three recipient strains, thus the effect of zinc on conjugation frequencies seemed to be concentration and strain dependent. Furthermore, differences in growth rates due to plasmid carriage were observed for one of the Salmonella strains.