Safety Properties of Escherichia coli O157:H7 Specific Bacteriophages: Recent Advances for Food Safety

• Published in: Foods 2023-11, Vol.12 (21), p.3989
• Main Authors: Oluwarinde, Bukola Opeyemi, Ajose, Daniel Jesuwenu, Abolarinwa, Tesleem Olatunde, Montso, Peter Kotsoana, Du Preez, Ilse, Njom, Henry Akum, Ateba, Collins Njie
• Format: Article
• Language: English
• Subjects: Animals; Antibiotics; Antigenic determinants; Antigens; Antimicrobial agents; antimicrobial resistance; Bacteria; Biological control; Contamination; Control methods; Drug resistance; E coli; Escherichia coli; Escherichia coli O157:H7; food borne infection; Food chains; Food contamination; Food contamination & poisoning; Food safety; Foodborne diseases; Foodborne pathogens; Genomics; Illnesses; Infections; one health approach; Pathogens; phage therapy; Phages; Pollution control; Public health; Safety; Surveillance; Toxins; Virulence; whole genome sequencing
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12213989

Shiga-toxin-producing Escherichia coli (STEC) is typically detected on food products mainly due to cross-contamination with faecal matter. The serotype O157:H7 has been of major public health concern due to the severity of illness caused, prevalence, and management. In the food chain, the main methods of controlling contamination by foodborne pathogens often involve the application of antimicrobial agents, which are now becoming less efficient. There is a growing need for the development of new approaches to combat these pathogens, especially those that harbour antimicrobial resistant and virulent determinants. Strategies to also limit their presence on food contact surfaces and food matrices are needed to prevent their transmission. Recent studies have revealed that bacteriophages are useful non-antibiotic options for biocontrol of E. coli O157:H7 in both animals and humans. Phage biocontrol can significantly reduce E. coli O157:H7, thereby improving food safety. However, before being certified as potential biocontrol agents, the safety of the phage candidates must be resolved to satisfy regulatory standards, particularly regarding phage resistance, antigenic properties, and toxigenic properties. In this review, we provide a general description of the main virulence elements of E. coli O157:H7 and present detailed reports that support the proposals that phages infecting E. coli O157:H7 are potential biocontrol agents. This paper also outlines the mechanism of E. coli O157:H7 resistance to phages and the safety concerns associated with the use of phages as a biocontrol.