A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles

Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disea...

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Bibliographic Details
Published in:Stem cell research 2019-08, Vol.39, p.101522-101522, Article 101522
Main Authors: Díaz-Guerra, Eva, Moreno-Jiménez, Elena P., de Rojas, Itziar, Rodríguez, César, Rodríguez-Traver, Eva, Arribas-González, Esther, Orera, María, Hernández, Isabel, Ruiz, Agustín, Vicario, Carlos
Format: Article
Language:English
Subjects:
Apolipoproteins E - genetics
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Line
Embryoid Bodies - cytology
Genotyping Techniques - methods
Humans
Immunohistochemistry
Karyotyping
Microsatellite Repeats - genetics
Reverse Transcriptase Polymerase Chain Reaction
Sendai virus - genetics
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Summary:Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2019.101522