Comparative Metabolome and Transcriptome Analysis of Anthocyanin Biosynthesis in White and Pink Petals of Cotton (Gossypium hirsutum L.)

Upland cotton (Gossypium hirsutum L.) is one of the important fiber crops. Cotton flowers usually appear white (or cream-colored) without colored spots at the petal base, and turn pink on the next day after flowering. In this study, using a mutant showing pink petals with crimson spots at their base...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-09, Vol.23 (17), p.10137
Main Authors: Shao, Dongnan, Liang, Qian, Wang, Xuefeng, Zhu, Qian-Hao, Liu, Feng, Li, Yanjun, Zhang, Xinyu, Yang, Yonglin, Sun, Jie, Xue, Fei
Format: Article
Language:English
Subjects:
Accumulation
Anthocyanins
Biosynthesis
Coloration
Cotton
Flavonoids
Flowering
Flowers
Flowers & plants
Genes
Glucosides
Gossypium hirsutum
Gossypium hirsutum L
Metabolites
metabolome
Mutants
petal
Petals
Pigments
Proteins
RNA-seq
Transcription factors
Transcriptomes
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Summary:Upland cotton (Gossypium hirsutum L.) is one of the important fiber crops. Cotton flowers usually appear white (or cream-colored) without colored spots at the petal base, and turn pink on the next day after flowering. In this study, using a mutant showing pink petals with crimson spots at their base, we conducted comparative metabolome and transcriptome analyses to investigate the molecular mechanism of coloration in cotton flowers. Metabolic profiling showed that cyanidin-3-O-glucoside and glycosidic derivatives of pelargonidins and peonidins are the main pigments responsible for the coloration of the pink petals of the mutant. A total of 2443 genes differentially expressed (DEGs) between the white and pink petals were identified by RNA-sequencing. Many DEGs are structural genes and regulatory genes of the anthocyanin biosynthesis pathway. Among them, MYB21, UGT88F3, GSTF12, and VPS32.3 showed significant association with the accumulation of cyanidin-3-O-glucoside in the pink petals. Taken together, our study preliminarily revealed the metabolites responsible for the pink petals and the key genes regulating the biosynthesis and accumulation of anthocyanins in the pink petals. The results provide new insights into the biochemical and molecular mechanism underlying anthocyanin biosynthesis in upland cotton.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms231710137