A comparative study of MassARRAY and GeneXpert assay in detecting rifampicin resistance in tuberculosis patients' clinical specimens

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potent tool for detecting drug resistance in tuberculosis (TB); however, concerns about its reliability have been raised. In this study, we assessed the reliability of MassARRAY (Sequenom, In...

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Bibliographic Details
Published in:Frontiers in microbiology 2024-02, Vol.15, p.1287806-1287806
Main Authors: Liang, Ruixia, Li, Jiankang, Zhao, Yue, Qi, Haoran, Bao, Shengjuan, Wang, Fen, Duan, Hongfei, Huang, Hairong
Format: Article
Language:English
Subjects:
drug resistance
MALDI-TOF MS
MassARRAY
Microbiology
reliability
tuberculosis
Xpert assay
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Summary:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potent tool for detecting drug resistance in tuberculosis (TB); however, concerns about its reliability have been raised. In this study, we assessed the reliability of MassARRAY (Sequenom, Inc.), which is a MALDI-TOF MS-based method, by comparing it to the well-established GeneXpert assay (Cepheid) as a reference method. A retrospective study was conducted using laboratory data retrieved from Henan Chest Hospital (Zhengzhou, China). To ensure a rigorous evaluation, we adopted a comprehensive assessment approach by integrating multiple outcomes of the Xpert assay across various specimen types. Among the 170 enrolled TB cases, MassARRAY demonstrated significantly higher sensitivity (85.88%, 146 of 170) compared to the Xpert assay (76.62%, 118 of 154) in TB diagnosis (  
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2024.1287806