Differentiation of Hedyotis diffusa and Common Adulterants Based on Chloroplast Genome Sequencing and DNA Barcoding Markers

Chinese herbal tea, also known as Liang Cha or cooling beverage, is popular in South China. It is regarded as a quick-fix remedy to relieve minor health problems. Willd. (colloquially Baihuasheshecao) is a common ingredient of cooling beverages. is also used to treat cancer and bacterial infections....

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Bibliographic Details
Published in:Plants (Basel) 2021-01, Vol.10 (1), p.161
Main Authors: Yik, Mavis Hong-Yu, Kong, Bobby Lim-Ho, Siu, Tin-Yan, Lau, David Tai-Wai, Cao, Hui, Shaw, Pang-Chui
Format: Article
Language:English
Subjects:
Adulterants
authentication
Bacterial diseases
Beverages
Biomarkers
Chloroplast DNA
chloroplast genome
Chloroplasts
Cooling
Cultural heritage
Deoxyribonucleic acid
Divergence
DNA
DNA barcode
DNA sequencing
Genes
Genetic engineering
Genomes
Health problems
Hedyotis
Herbal medicine
Herbal tea
Microsatellites
Morphology
ndhD
Next-generation sequencing
Nucleotide sequence
Photosynthesis
Phylogenetics
Physical characteristics
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Summary:Chinese herbal tea, also known as Liang Cha or cooling beverage, is popular in South China. It is regarded as a quick-fix remedy to relieve minor health problems. Willd. (colloquially Baihuasheshecao) is a common ingredient of cooling beverages. is also used to treat cancer and bacterial infections. Owing to the high demand for , two common adulterants, (DC.) Sivar and Biju (colloquially Nidingjingcao) and (L.) Lam. (colloquially Shuixiancao), are commonly encountered in the market. Owing to the close similarity of their morphological characteristics, it is difficult to differentiate them. Here, we sequenced the complete chloroplast genomes of the three species of using next-generation sequencing (NGS). By comparing the complete chloroplast genomes, we found that they are closely related in the subfamily Rubioideae. We also discovered that there are significant differences in the number and repeating motifs of microsatellites and complex repeats and revealed three divergent hotspots, intergenic spacer, and . By using these species-specific sequences, we propose new DNA barcoding markers for the authentication of and its two common adulterants.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10010161