In vitro triple coculture with gut microbiota from spondyloarthritis patients is characterized by inter-individual differences in inflammatory responses

Spondyloarthritis is a group of chronic inflammatory diseases that primarily affects axial or peripheral joints and is frequently associated with inflammation at non-articular sites. The disease is multifactorial, involving genetics, immunity and environmental factors, including the gut microbiota....

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Published in:Scientific reports 2022-06, Vol.12 (1), p.10475-10475, Article 10475
Main Authors: Beterams, Annelore, Calatayud Arroyo, Marta, De Paepe, Kim, De Craemer, Ann-Sophie, Elewaut, Dirk, Venken, Koen, Van de Wiele, Tom
Format: Article
Language:English
Subjects:
631/326/41/2533
692/4023/1670/2766/1827
Arthritis
Cytokines
Disease
Environmental factors
Epithelial cells
Flow cytometry
Genetics
Humanities and Social Sciences
Inflammatory diseases
Intestinal microflora
Joint diseases
Microbiomes
Microbiota
multidisciplinary
Phylogeny
Rheumatic diseases
Science
Science (multidisciplinary)
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Summary:Spondyloarthritis is a group of chronic inflammatory diseases that primarily affects axial or peripheral joints and is frequently associated with inflammation at non-articular sites. The disease is multifactorial, involving genetics, immunity and environmental factors, including the gut microbiota. In vivo , microbiome contributions are difficult to assess due to the multifactorial disease complexity. In a proof-of-concept approach, we therefore used a triple coculture model of immune-like, goblet and epithelial cells to investigate whether we could detect a differential impact from spondyloarthritis- vs. healthy-derived gut microbiota on host cell response. Despite their phylogenetic resemblance, flow cytometry-based phenotypic clustering revealed human-derived gut microbiota from healthy origin to cluster together and apart from spondyloarthritis donors. At host level, mucus production was higher upon exposure to healthy microbiota. Pro-inflammatory cytokine responses displayed more inter-individual variability in spondyloarthritis than in healthy donors. Interestingly, the high dominance in the initial sample of one patient of Prevotella , a genus previously linked to spondyloarthritis, resulted in the most differential host response upon 16 h host-microbe coincubation. While future research should further focus on inter-individual variability by using gut microbiota from a large cohort of patients, this study underscores the importance of the gut microbiota during the SpA disease course.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-13582-7