Molecular glues modulate protein functions by inducing protein aggregation: A promising therapeutic strategy of small molecules for disease treatment

• Published in: Acta pharmaceutica Sinica. B 2022-09, Vol.12 (9), p.3548-3566
• Main Authors: Wu, Hongyu, Yao, Hong, He, Chen, Jia, Yilin, Zhu, Zheying, Xu, Shengtao, Li, Dahong, Xu, Jinyi
• Format: Article
• Language: English
• Subjects: Dimerization; Molecular glue; Polymerization; Protein–protein interaction; Review; Small molecule
• ISSN: 2211-3835; 2211-3843
• DOI: 10.1016/j.apsb.2022.03.019

Molecular glues can specifically induce aggregation between two or more proteins to modulate biological functions. In recent years, molecular glues have been widely used as protein degraders. In addition, however, molecular glues play a variety of vital roles, such as complex stabilization, interactome modulation and transporter inhibition, enabling challenging therapeutic targets to be druggable and offering an exciting novel approach for drug discovery. Since most molecular glues are identified serendipitously, exploration of their systematic discovery and rational design are important. In this review, representative examples of molecular glues with various physiological functions are divided into those mediating homo-dimerization, homo-polymerization and hetero-dimerization according to their aggregation modes, and we attempt to elucidate their mechanisms of action. In particular, we aim to highlight some biochemical techniques typically exploited within these representative studies and classify them in terms of three stages of molecular glue development: starting point, optimization and identification. Through rational design, optimization and identification, molecular glues can specifically induce proteins’ homo-dimerization, homo-polymerization or hetero-dimerization to regulate their functions, which is a potential therapeutic strategy. [Display omitted]