Title Changes in the Mineral Composition of Rat Femoral Bones Induced by Implantation of LNCaP Prostate Cancer Cells and Dietary Supplementation

• Published in: Nutrients 2020-12, Vol.13 (1), p.100
• Main Authors: Skrajnowska, Dorota, Jagielska, Agata, Ruszczyńska, Anna, Wagner, Barbara, Bielecki, Wojciech, Bobrowska-Korczak, Barbara
• Format: Article
• Language: English
• Subjects: Angiogenesis; Animal tissues; Biomedical materials; Bone composition; Bone growth; Bone matrix; bone mineral loss; Bone resorption; Bones; Breast; Calcium; Calcium (dietary); cancer; Cell cycle; Cobalt; Composition; Deoxyribonucleic acid; Dietary intake; Dietary supplements; DNA; Femur; Homeostasis; Hydroxyapatite; Implantation; Iron; Kruskal-Wallis test; LNCaP implantation in rats; Manganese; Metabolism; Metastases; Metastasis; Mineral composition; Minerals; Molybdenum; Nutrition research; Osteolysis; Osteoporosis; Principal components analysis; Prostate cancer; RNA polymerase; Strontium; supplementation with minerals; Surgical implants; Testosterone; Trace elements; Transplants & implants; Tumors; Zinc
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13010100

Prostate cancer (PCa) is the second most frequent cancer in men and the fifth most common cause of death worldwide, with an estimated 378,553 deaths in 2020. Prostate cancer shows a strong tendency to form metastatic foci in the bones. A number of interactions between cancer cells attacking bones and cells of the bone matrix lead to destruction of the bone and growth of the tumour. The last few decades have seen increased interest in the precise role of minerals in human health and disease. Tumour cells accumulate various minerals that promote their intensive growth. Bone, as a storehouse of elements, can be a valuable source of them for the growing tumour. There are also reports suggesting that the presence of some tumours, e.g., of the breast, can adversely affect bone structure even in the absence of metastasis to this organ. This paper presents the effect of chronic dietary intake of calcium, iron and zinc, administered in doses corresponding maximally to twice their level in a standard diet, on homeostasis of selected elements (Ca, K, Zn, Fe, Cu, Sr, Ni, Co, Mn and Mo) in the femoral bones of healthy rats and rats with implanted cancer cells of the LNCaP line. The experiment was conducted over 90 days. After the adaptation period, the animals were randomly divided into four dietary groups: standard diet and supplementation with Zn, Fe and Ca. Every dietary group was divided into experimental group (with implanted cancer cells) and control group (without implanted cancer cells). The cancer cells (LnCaP) were implanted intraperitoneally in the amount 1 × 10 to the rats at day 90 of their lifetime. Bone tissue was dried and treated with microwave-assisted mineral digestation. Total elemental content was quantified by ICP-MS. Student's -test and Anova or Kruskal-Wallis tests were applied in order to compare treatment and dietary groups. In the case of most of the diets, especially the standard diet, the femoral bones of rats with implanted LNCaP cells showed a clear downward trend in the content of the elements tested, which may be indicative of slow osteolysis taking place in the bone tissue. In the group of rats receiving the standard diet, there were significant reductions in the content of Mo (by 83%), Ca (25%), Co (22%), Mn (13%), K (13%) and Sr (9%) in the bone tissue of rats with implanted LNCaP cells in comparison with the control group receiving the same diet but without LNCaP implantation. Supplementation of the rat diet with calcium, zinc and i