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Deletion of the L7L-L11L Genes Attenuates ASFV and Induces Protection against Homologous Challenge

African swine fever (ASF), caused by the African swine fever virus (ASFV), is a major epidemic disease endangering the swine industry. Although a number of vaccine candidates have been reported, none are commercially available yet. To explore the effect of unknown genes on the biological characteris...

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Published in:Viruses 2021-02, Vol.13 (2), p.255
Main Authors: Zhang, Jingyuan, Zhang, Yanyan, Chen, Teng, Yang, Jinjin, Yue, Huixian, Wang, Lidong, Zhou, Xintao, Qi, Yu, Han, Xun, Ke, Junnan, Wang, Shuchao, Yang, Jinmei, Miao, Faming, Zhang, Shoufeng, Zhang, Fei, Wang, Ying, Li, Min, Hu, Rongliang
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cited_by cdi_FETCH-LOGICAL-c469t-7496b19a9d299d526ad0138f158a770ad9ac17bc66460e072a16462a02fb34803
cites cdi_FETCH-LOGICAL-c469t-7496b19a9d299d526ad0138f158a770ad9ac17bc66460e072a16462a02fb34803
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container_issue 2
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container_title Viruses
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creator Zhang, Jingyuan
Zhang, Yanyan
Chen, Teng
Yang, Jinjin
Yue, Huixian
Wang, Lidong
Zhou, Xintao
Qi, Yu
Han, Xun
Ke, Junnan
Wang, Shuchao
Yang, Jinmei
Miao, Faming
Zhang, Shoufeng
Zhang, Fei
Wang, Ying
Li, Min
Hu, Rongliang
description African swine fever (ASF), caused by the African swine fever virus (ASFV), is a major epidemic disease endangering the swine industry. Although a number of vaccine candidates have been reported, none are commercially available yet. To explore the effect of unknown genes on the biological characteristics of ASFV and the possibility of a gene-deleted isolate as a vaccine candidate, the strain SY18ΔL7-11, with deletions of L7L-L11L genes from ASFV SY18, was constructed, and its biological properties were analyzed. The results show that deletion of genes L7L-L11L did not affect replication of the virus in vitro. Virulence of SY18△L7-11 was significantly reduced, as 11 of the 12 pigs survived for 28 days after intramuscular inoculation with a low dose (10 TCID ) or a high dose (10 TCID ) of SY18ΔL7-11. All 11 surviving pigs were completely protected against challenge with the parental ASFV SY18 on 28 days postinoculation (dpi). Transient fever and/or irregularly low levels of genomic DNA in the blood were monitored in some pigs after inoculation. No ASF clinical signs or viremia were monitored after challenge. Antibodies to ASFV were induced in all pigs from 14 to 21 days postinoculation. IFN-γ was detected in most of the inoculated pigs, which is usually inhibited in ASFV-infected pigs. Overall, the results demonstrate that SY18ΔL7-11 is a candidate for further constructing safer vaccine(s), with better joint deletions of other gene(s) related to virulence.
doi_str_mv 10.3390/v13020255
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Although a number of vaccine candidates have been reported, none are commercially available yet. To explore the effect of unknown genes on the biological characteristics of ASFV and the possibility of a gene-deleted isolate as a vaccine candidate, the strain SY18ΔL7-11, with deletions of L7L-L11L genes from ASFV SY18, was constructed, and its biological properties were analyzed. The results show that deletion of genes L7L-L11L did not affect replication of the virus in vitro. Virulence of SY18△L7-11 was significantly reduced, as 11 of the 12 pigs survived for 28 days after intramuscular inoculation with a low dose (10 TCID ) or a high dose (10 TCID ) of SY18ΔL7-11. All 11 surviving pigs were completely protected against challenge with the parental ASFV SY18 on 28 days postinoculation (dpi). Transient fever and/or irregularly low levels of genomic DNA in the blood were monitored in some pigs after inoculation. No ASF clinical signs or viremia were monitored after challenge. Antibodies to ASFV were induced in all pigs from 14 to 21 days postinoculation. IFN-γ was detected in most of the inoculated pigs, which is usually inhibited in ASFV-infected pigs. Overall, the results demonstrate that SY18ΔL7-11 is a candidate for further constructing safer vaccine(s), with better joint deletions of other gene(s) related to virulence.</description><identifier>ISSN: 1999-4915</identifier><identifier>EISSN: 1999-4915</identifier><identifier>DOI: 10.3390/v13020255</identifier><identifier>PMID: 33567491</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>African swine fever ; African Swine Fever - prevention &amp; control ; African swine fever virus ; African Swine Fever Virus - genetics ; African Swine Fever Virus - immunology ; African Swine Fever Virus - pathogenicity ; Animals ; Antibodies, Viral - blood ; Asfarviridae ; Blood ; Bone marrow ; Cells, Cultured ; Cytokines ; deletion ; Deoxyribonucleic acid ; DNA ; Experiments ; Gene Deletion ; Genes ; Genes, Viral - genetics ; Genomes ; Hogs ; Injections, Intramuscular ; Inoculation ; Interferon-gamma - blood ; L7L-L11L genes ; Macrophages - virology ; Monoclonal antibodies ; Polymerase chain reaction ; Swine ; vaccine candidate ; Vaccines ; Vaccines, Attenuated - administration &amp; dosage ; Vaccines, Attenuated - genetics ; Veterinary medicine ; Viral Vaccines - administration &amp; dosage ; Viral Vaccines - genetics ; Viremia ; Virulence ; Virulence - genetics ; Viruses ; γ-Interferon</subject><ispartof>Viruses, 2021-02, Vol.13 (2), p.255</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). 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Zhang, Yanyan ; Chen, Teng ; Yang, Jinjin ; Yue, Huixian ; Wang, Lidong ; Zhou, Xintao ; Qi, Yu ; Han, Xun ; Ke, Junnan ; Wang, Shuchao ; Yang, Jinmei ; Miao, Faming ; Zhang, Shoufeng ; Zhang, Fei ; Wang, Ying ; Li, Min ; Hu, Rongliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-7496b19a9d299d526ad0138f158a770ad9ac17bc66460e072a16462a02fb34803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>African swine fever</topic><topic>African Swine Fever - prevention &amp; control</topic><topic>African swine fever virus</topic><topic>African Swine Fever Virus - genetics</topic><topic>African Swine Fever Virus - immunology</topic><topic>African Swine Fever Virus - pathogenicity</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Asfarviridae</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>deletion</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Experiments</topic><topic>Gene Deletion</topic><topic>Genes</topic><topic>Genes, Viral - genetics</topic><topic>Genomes</topic><topic>Hogs</topic><topic>Injections, Intramuscular</topic><topic>Inoculation</topic><topic>Interferon-gamma - blood</topic><topic>L7L-L11L genes</topic><topic>Macrophages - virology</topic><topic>Monoclonal antibodies</topic><topic>Polymerase chain reaction</topic><topic>Swine</topic><topic>vaccine candidate</topic><topic>Vaccines</topic><topic>Vaccines, Attenuated - administration &amp; 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Although a number of vaccine candidates have been reported, none are commercially available yet. To explore the effect of unknown genes on the biological characteristics of ASFV and the possibility of a gene-deleted isolate as a vaccine candidate, the strain SY18ΔL7-11, with deletions of L7L-L11L genes from ASFV SY18, was constructed, and its biological properties were analyzed. The results show that deletion of genes L7L-L11L did not affect replication of the virus in vitro. Virulence of SY18△L7-11 was significantly reduced, as 11 of the 12 pigs survived for 28 days after intramuscular inoculation with a low dose (10 TCID ) or a high dose (10 TCID ) of SY18ΔL7-11. All 11 surviving pigs were completely protected against challenge with the parental ASFV SY18 on 28 days postinoculation (dpi). Transient fever and/or irregularly low levels of genomic DNA in the blood were monitored in some pigs after inoculation. No ASF clinical signs or viremia were monitored after challenge. Antibodies to ASFV were induced in all pigs from 14 to 21 days postinoculation. IFN-γ was detected in most of the inoculated pigs, which is usually inhibited in ASFV-infected pigs. Overall, the results demonstrate that SY18ΔL7-11 is a candidate for further constructing safer vaccine(s), with better joint deletions of other gene(s) related to virulence.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33567491</pmid><doi>10.3390/v13020255</doi><orcidid>https://orcid.org/0000-0001-9782-9656</orcidid><oa>free_for_read</oa></addata></record>
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subjects African swine fever
African Swine Fever - prevention & control
African swine fever virus
African Swine Fever Virus - genetics
African Swine Fever Virus - immunology
African Swine Fever Virus - pathogenicity
Animals
Antibodies, Viral - blood
Asfarviridae
Blood
Bone marrow
Cells, Cultured
Cytokines
deletion
Deoxyribonucleic acid
DNA
Experiments
Gene Deletion
Genes
Genes, Viral - genetics
Genomes
Hogs
Injections, Intramuscular
Inoculation
Interferon-gamma - blood
L7L-L11L genes
Macrophages - virology
Monoclonal antibodies
Polymerase chain reaction
Swine
vaccine candidate
Vaccines
Vaccines, Attenuated - administration & dosage
Vaccines, Attenuated - genetics
Veterinary medicine
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viremia
Virulence
Virulence - genetics
Viruses
γ-Interferon
title Deletion of the L7L-L11L Genes Attenuates ASFV and Induces Protection against Homologous Challenge
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