Determination of the fate of Cholecalciferol injected by the basis of 25D3 plasma concentration

• Published in: Scientific reports 2023-03, Vol.13 (1), p.4094-4094, Article 4094
• Main Authors: Mousavi Rad, Seyed Ali, Haji Hajikolaei, Mohammad Rahim, Nouri, Mohammad, Seyfi Abad Shapouri, Masoud Reza, Ghorbanpoor, Masoud
• Format: Article
• Language: English
• Subjects: 631/443; 631/45; 692/163; Animals; Cattle; Cholecalciferol - pharmacology; Dietary Supplements; Digestive system; Endocrine system; Farm buildings; Humanities and Social Sciences; Injection; multidisciplinary; Nutrient deficiency; Plasma; Science; Science (multidisciplinary); Solar radiation; Sun; Variation; Vitamin D; Vitamin D Deficiency; Vitamins - therapeutic use
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-29870-9

Sun exposure in bovines is believed to be the most important route of 25D 3 synthesis in suitable latitudes. In some situations, e.g. breeding systems, solar radiation cannot reach or penetrate into the skin and thus causes the 25D 3 deficiency. Because of the critical effect of vitamin D on the immune and endocrine systems, the plasma must be enriched with 25D 3 in a short period of time. In such a condition, injection of Cholecalciferol has been recommended. However, to our knowledge, the certain dose of Cholecalciferol injection for rapid 25D 3 plasma enrichment has not been verified. On the other hand, it seems that the basis 25D 3 concentration can influence or shift the 25D 3 metabolism at the injection time. In the same line, the present study, designed to induce the different basis 25D 3 concentration in treatment groups, aimed at investigating the effect of Cholecalciferol intramuscularly injection with the intermediate dose (11,000 IU/kg) on the calves' plasma 25D 3 with different basis 25D 3 . Besides, an attempt was made to clarify the time that 25D 3 reaches the sufficient concentration after injection in different treatment groups. To do this, twenty calves of 3 to 4 months old were chosen for the farm with semi-industrial elements. Furthermore, the effect of optional sun exposure/deprivation and Cholecalciferol injection on the 25D 3 concentration variations was assayed. To do this, the calves were divided into four groups. Groups A and B were unconstrained to choose sun to expose or shadow in a semi-roofed place, but groups C and D were restricted to the completely dark barn. The interference of the digestive system in supplying vitamin D was minimized through dietary. All groups had a different basic concentration (25D 3 ) on the day 21 of the experiment. At this time, groups A and C received the intermediate dose of (11,000 IU/kg) Cholecalciferol intramuscularly (IM). After Cholecalciferol injection, the effects of basis 25D 3 concentration on the details of variation and fate of plasma concentration of 25D 3 were investigated. The data collected from the two groups C and D showed that sun deprivation without any vitamin D supplementation, could rapidly and severely deplete the plasma from 25D 3 . Cholecalciferol injection could not immediately increase the 25D 3 in the groups C and A. However, this injection enriches the 25D 3 to sufficient value after two weeks if the basis 25D 3 of plasma is insufficient, i.e. less than 30 ng/mL. Moreo