A novel structurally identified epitope delivered by macrophage membrane-coated PLGA nanoparticles elicits protection against Pseudomonas aeruginosa

The increasing prevalence of antibiotic resistance by Pseudomonas aeruginosa (PA) raises an urgent need for an effective vaccine. The outer membrane proteins of PA, especially those that are upregulated during infection, are ideal vaccine targets. However, the strong hydrophobicity of these proteins...

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Bibliographic Details
Published in:Journal of nanobiotechnology 2022-12, Vol.20 (1), p.532-14, Article 532
Main Authors: Gao, Chen, Chen, Yin, Cheng, Xin, Zhang, Yi, Zhang, Yueyue, Wang, Ying, Cui, Zhiyuan, Liao, Yaling, Luo, Ping, Wu, Weihui, Wang, Cheng, Zeng, Hao, Zou, Quanming, Gu, Jiang
Format: Article
Language:English
Subjects:
Animals
Antibiotic resistance
Antibiotics
Antibodies
Antigenic determinants
Antigens
Bacterial proteins
Bacterial vaccines
Chronic obstructive pulmonary disease
Drug delivery systems
Drug resistance
Drugs
E coli
Epitope-based vaccine
Epitopes
Glycolic acid
Health care
Hydrophobicity
Immune response
Immune system
Immunogenicity
Infections
Laboratory animals
Lymphocytes T
Macrophages
Membrane Proteins
Membranes
Mice
Nanoparticle
Nanoparticles
Nosocomial infections
Outer membrane protein
Outer membrane proteins
Pathogens
Peptides
Pharmaceutical research
Physiological aspects
Polylactide-co-glycolide
Polyvinyl alcohol
Prevention
Production processes
Proteins
Pseudomonas aeruginosa
Pseudomonas aeruginosa infections
Pseudomonas Infections - prevention & control
Streptococcus infections
Vaccines
Vehicles
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Summary:The increasing prevalence of antibiotic resistance by Pseudomonas aeruginosa (PA) raises an urgent need for an effective vaccine. The outer membrane proteins of PA, especially those that are upregulated during infection, are ideal vaccine targets. However, the strong hydrophobicity of these proteins hinders their application for this purpose. In this study, we selected eight outer membrane proteins from PA with the most significantly upregulated expression. Their extracellular loops were analyzed and screened by using sera from patients who had recovered from PA infection. As a result, a novel immunogenic epitope (Ep ) from PilY1 (PA4554) was found. Moreover, we constructed a macrophage membrane-coated PLGA (poly lactic-co-glycolic acid) nanoparticle vaccine carrying PilY1 Ep (PNPs@M-Ep ) that elicits a Th2 immune response and confers adequate protection in mice. Our data furnished the promising vaccine candidate PNPs@M-Ep while providing additional evidence for structure-based epitope identification and vaccine design.
ISSN:1477-3155
1477-3155
DOI:10.1186/s12951-022-01725-x