Anti-Osteogenic Activity of Cadmium in Zebrafish

Among the many anthropogenic chemicals that end up in the aquatic ecosystem, heavy metals, in particular cadmium, are hazardous compounds that have been shown to affect developmental, reproductive, hepatic, hematological, and immunological functions in teleost fish. There is also evidence that cadmi...

Full description

Saved in:
Bibliographic Details
Published in:Fishes 2019, Vol.4 (1), p.11-0
Main Authors: Tarasco, Marco, Cardeira, João, Viegas, Michael, Caria, Joana, Martins, Gil, Gavaia, Paulo, Cancela, M., Laizé, Vincent
Format: Article
Language:English
Subjects:
Anthropogenic factors
Aquatic ecosystems
Biological fertilization
Bone growth
Bones
Cadmium
caudal fin regeneration
Chloride
Danio rerio
Drinking water
Eggs
Fertilization
Fins
Fish
Food contamination & poisoning
Freshwater fishes
gene expression
Genes
Heavy metals
Larvae
Malformations
Mammals
Mechanical properties
Metals
operculum growth
Osteoblastogenesis
Osteogenesis
Osteoporosis
osteotoxicity
Pollutants
Protein kinase A
signaling pathways
skeletal deformities
Vertebrae
Zebrafish
zebrafish Danio rerio
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among the many anthropogenic chemicals that end up in the aquatic ecosystem, heavy metals, in particular cadmium, are hazardous compounds that have been shown to affect developmental, reproductive, hepatic, hematological, and immunological functions in teleost fish. There is also evidence that cadmium disturbs bone formation and skeletal development, but data is scarce. In this work, zebrafish was used to further characterize the anti-osteogenic/osteotoxic effects of cadmium and gain insights into underlying mechanisms. Upon exposure to cadmium, a reduction of the opercular bone growth was observed in 6-days post-fertilization (dpf) larvae and an increase in the incidence of skeletal deformities was evidenced in 20-dpf post-larvae. The extent and stiffness of newly formed bone was also affected in adult zebrafish exposed to cadmium while regenerating their caudal fin. A pathway reporter assay revealed a possible role of the MTF-1 and cAMP/PKA signaling pathways in mechanisms of cadmium osteotoxicity, while the expression of genes involved in osteoblast differentiation and matrix production was strongly reduced in cadmium-exposed post-larvae. This work not only confirmed cadmium anti-osteogenic activity and identified targeted pathways and genes, but it also suggested that cadmium may affect biomechanical properties of bone.
ISSN:2410-3888
2410-3888
DOI:10.3390/fishes4010011