The expression of the GATA6 gene in oral carcinoma cell lines

This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. In this study, we sub-cultured normal oral epithelial cell lines HOK, human tongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and human salivary gland adenoid cystic carcinoma cell lines...

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Bibliographic Details
Published in:World journal of surgical oncology 2021-05, Vol.19 (1), p.153-153, Article 153
Main Authors: Xu, Cheng-Lin, Guan, Wei-Qun, Wang, Xue-Ying
Format: Article
Language:English
Subjects:
Adenoid
Biotechnology
Cancer
Carcinoma, Adenoid Cystic
Carcinoma, Squamous Cell - genetics
Cell Line, Tumor
Epithelial cells
Epithelium
GATA6
Gata6 gene
GATA6 Transcription Factor - genetics
Gene expression
Genes
Genetic aspects
Health aspects
Humans
Laryngeal cancer
Lymphatic system
Metastasis
Mouth cancer
Normal distribution
Oral cancer
Oral carcinoma
Oral epithelial cell
Polymerase chain reaction
Prognosis
Protein expression
Proteins
Reverse transcription
RT-PCR
Salivary gland
Salivary Gland Neoplasms
Salivary glands
Squamous cell carcinoma
Statistical analysis
Tongue
Tongue Neoplasms - genetics
Tumor cell lines
Tumors
Western blot
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Summary:This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. In this study, we sub-cultured normal oral epithelial cell lines HOK, human tongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and human salivary gland adenoid cystic carcinoma cell lines SACC-LM and SACC-83. Subsequently, we used reverse transcription-polymerase chain reaction RT-PCR and Western blot methods to detect the mRNA and the protein expressions of GATA6 in normal oral epithelial cells, human tongue squamous cell carcinoma cells, and human salivary gland adenoid cystic carcinoma cells. The results of this study showed that the mRNA expression levels of GATA6 in CAL-27, SCC-4, and SACC-LM cells were significantly increased when compared with the HOK cells. However, the mRNA expression level of GATA6 in the SACC-83 cells had no significant difference compared with the HOK cells. The protein expression levels of GATA6 in the SCC-4 and SACC-LM cells were, however, significantly increased whereas the protein expression levels of GATA6 in the CAL-27 and SACC-83 cells had no significant difference when compared with the HOK cells. The GATA6 gene may be related to the occurrence and progression of certain oral cancers.
ISSN:1477-7819
1477-7819
DOI:10.1186/s12957-021-02245-y