Synthesis of UiO-67 Metal-organic Frameworks (MOFs) and their Application as Antibacterial and Anticancer Materials

• Published in: Journal of pure & applied microbiology : an international research journal of microbiology 2024-09, Vol.18 (3), p.1824-1837
• Main Authors: Almotiry, Sitah, Alqriqri, Mehal, Alhogbi, Basma, Abo-Aba, Salah E.M, Jaremko, Mariusz, Salam, Mohamed Abdel
• Format: Article
• Language: English
• Subjects: antibacterial; Antibacterial agents; anticancer; Bacteria; Cancer; Care and treatment; Drug therapy; hydrothermal technique; Infrared spectroscopy; Liver cancer; uio-67
• ISSN: 0973-7510; 2581-690X
• DOI: 10.22207/JPAM.18.3.30

This study involved the synthesis of the UiO-67 metal-organic framework; UiO-67 is a well-known type of MOF obtained by coordinating the Zr6O4(OH)4 metal unit with the 4,42-biphenyldicarboxylate organic linker, using the hydrothermal technique. The novelty of the current work is to synthesize UiO-67 MOFs, and their application as biological agents for antibacterial and cancer cells. Subsequently, the composite material UiO-67 was subjected to a comprehensive characterization process involving Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermal gravimetric analyses (TGA) and surface area analysis, and the results showed the successful synthesis of the UiO-67 MOFs, with a high specific surface area of 1415 m2/g. The­­­ synthesized UiO-67 for its antibacterial properties tested against five pathogenic bacterial strains, which include three gram-positive and methicillin-resistant pairs including MRSA, S. aureus and Enterococcus faecalis, and Two gram-negative bacteria E. colli and S. typhimurium using the agar well diffusion method. These findings have shown enhanced, strong antibacterial activity against all the five used gram-positive and gram-negative bacterial strains. Furthermore, the anticancer efficacy of UiO-67 was evaluated on two distinct types of cancer cells: We are using MCF-7 (human breast cancer cell line) and HepG2 (human liver cancer cells). The experiments prove that UiO-67 has the potential of cytotoxicity against both Glioblastoma and H460 cancer lines with the ability to inhibit apoptosis at the same time.