Polymorphism, expression and structure analysis of key genes in the ovarian steroidogenesis pathway in sheep (Ovis aries)

Background Litter size is an important factor that significantly affects the development of the sheep industry. Our previous TMT proteomics analysis found that three key proteins in the ovarian steroidogenesis pathway, STAR, HSD3B1, and CYP11A1, may affect the litter size trait of Small Tail Han she...

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Published in:Veterinary medicine and science 2021-07, Vol.7 (4), p.1303-1315
Main Authors: Hu, Wen‐Ping, Liu, Ming‐Qiu, Tian, Zhi‐Long, Liu, Qiu‐Yue, Zhang, Zhuang‐Biao, Tang, Ji‐Shun, He, Xiao‐Yun, Zhu, Yan‐Yan, Wang, Yuan‐Yuan, Chu, Ming‐Xing
Format: Article
Language:English
Subjects:
Association analysis
Bioinformatics
Breeding
Breeding of animals
CYP11A1
Cytochrome
Dehydrogenases
Enzymes
Gene expression
Gene polymorphism
Genetic diversity
Hormones
HSD3B1
Litter size
Mass spectrometry
Original
ovarian steroidogenesis pathway
Ovaries
Ovulation
Pituitary
Polymorphism
Proteins
Proteomics
Scientific imaging
sheep
Single-nucleotide polymorphism
STAR
Steroidogenesis
Steroids
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Summary:Background Litter size is an important factor that significantly affects the development of the sheep industry. Our previous TMT proteomics analysis found that three key proteins in the ovarian steroidogenesis pathway, STAR, HSD3B1, and CYP11A1, may affect the litter size trait of Small Tail Han sheep. Objective The purpose of this study was to better understand the relationship between polymorphisms of these three genes and litter size. Material and Method Sequenom MassARRAY detected genetic variance of the three genes in 768 sheep. Real‐time qPCR of the three genes was used to compare their expression in monotocous and polytocous sheep in relevant tissues. Finally, bioinformatics analysis predicted the protein sequences of the different SNP variants. Result Association analysis showed that there was a significant difference in litter size among the genotypes at two loci of the CYP11A1 gene (p  0.05). However, STAR expression was significantly different in polytocous and monotocous sheep in the pituitary (p 
ISSN:2053-1095
2053-1095
DOI:10.1002/vms3.485