Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform

Lab-on-a-disc (LOD) has emerged as a promising candidate for a point-of-care testing (POCT) device because it can effectively integrate complex fluid manipulation steps using multiple layers of polymeric substrates. However, it is still highly challenging to design and fabricate temperature measurem...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2019-07, Vol.19 (14), p.3207
Main Authors: Lim, Da Ye Seul, Seo, Moo-Jung, Yoo, Jae Chern
Format: Article
Language:English
Subjects:
Automation
Bacteria
Body Temperature
Colorimetry
convolutional neural network
Deoxyribonucleic acid
DNA
Food contamination & poisoning
Heating
Humans
hydroxy naphthol blue
Illnesses
Lab-On-A-Chip Devices
lab-on-a-disc
Laser beams
loop-mediated isothermal amplification
Methods
Neural networks
Neural Networks, Computer
Point-of-Care Testing
Researchers
Salmonella
Sensors
Temperature
Temperature control
Temperature measurement
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Summary:Lab-on-a-disc (LOD) has emerged as a promising candidate for a point-of-care testing (POCT) device because it can effectively integrate complex fluid manipulation steps using multiple layers of polymeric substrates. However, it is still highly challenging to design and fabricate temperature measurement and heating system in non-contact with the surface of LOD, which is a prerequisite to successful realization of DNA amplification especially with a rotatable disc. This study presents a Lab-on-a-disc (LOD)-based automatic loop-mediated isothermal amplification (LAMP) system, where a thermochromic coating (
ISSN:1424-8220
1424-8220
DOI:10.3390/s19143207