The adipose-derived mesenchymal stem cell secretome promotes hepatic regeneration in miniature pigs after liver ischaemia-reperfusion combined with partial resection

• Published in: Stem cell research & therapy 2021-03, Vol.12 (1), p.218-218, Article 218
• Main Authors: Jiao, Zhihui, Ma, Yajun, Zhang, Qianzhen, Wang, Yue, Liu, Tao, Liu, Xiaoning, Piao, Chenxi, Liu, Boyang, Wang, Hongbin
• Format: Article
• Language: English
• Subjects: Analysis; Angiogenesis; Antibodies; ASC-secretome; Cyclin D1; Cytokines; Extracellular matrix; Fibroblast growth factors; Gene expression; Growth factors; Hepatectomy; Hepatic regeneration; Hepatocytes; Hogs; IL-1β; Inflammation; Inflammatory response; Interleukin 10; Interleukin 6; Ischaemia-reperfusion; Ischemia; Laparoscopy; Liver; Liver diseases; Mesenchymal stem cells; Miniature pig; Paracrine signalling; Proliferating cell nuclear antigen; Proteins; Reagents; Reperfusion; RNA; Secretome; Stat3 protein; Stem cells; Surgery; Swine; Transforming growth factors; Transplantation; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vascular endothelial growth factor
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-021-02284-y

Hepatic ischaemia-reperfusion injury (HIRI) is inevitable in complicated liver surgery and is a major factor leading to postoperative complications and liver dysfunction. Studies have shown that the paracrine mechanisms of stem cell may be essential to tissue repair and functional improvement after transplantation. However, the role of the adipose-derived mesenchymal stem cell secretome (ASC-secretome) in liver regeneration in large animals remains to be determined. Twenty-four miniature pigs were subjected to laparoscopic liver ischaemia-reperfusion combined with partial hepatectomy and divided into the following four groups: the saline group, the DMEM group, the ASC group and the ASC-secretome group. Serum and liver tissue samples were collected before the operation and at 1, 3 and 7 days after the operation, and changes in tissue pathology, serum inflammation, liver function, angiogenesis-related factors and liver tissue regeneration-related genes and proteins were evaluated. Detailed histological analysis showed that ASCs and the ASC-secretome changed pathological damage to liver tissue after liver ischaemia-reperfusion combined with partial hepatectomy (1 and 3 days: p