Modeling Dynamics of Human Gut Microbiota Derived from Gluten Metabolism: Obtention, Maintenance and Characterization of Complex Microbial Communities

Western diets are rich in gluten-containing products, which are frequently poorly digested. The human large intestine harbors microorganisms able to metabolize undigested gluten fragments that have escaped digestion by human enzymatic activities. The aim of this work was obtaining and culturing comp...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-04, Vol.25 (7), p.4013
Main Authors: Carnicero-Mayo, Yaiza, Sáenz de Miera, Luis E, Ferrero, Miguel Ángel, Navasa, Nicolás, Casqueiro, Javier
Format: Article
Language:English
Subjects:
Actinobacteria
Bacteria
Clostridiales
Enzymes
Firmicutes
Gastrointestinal Microbiome
Gluten
gluten metabolism
Glutens
gut microbiota
Humans
in vitro digestion
Metabolism
microbial communities
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Nervous system
Phylogenetics
Physiological aspects
Taxonomy
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Summary:Western diets are rich in gluten-containing products, which are frequently poorly digested. The human large intestine harbors microorganisms able to metabolize undigested gluten fragments that have escaped digestion by human enzymatic activities. The aim of this work was obtaining and culturing complex human gut microbial communities derived from gluten metabolism to model the dynamics of healthy human large intestine microbiota associated with different gluten forms. For this purpose, stool samples from six healthy volunteers were inoculated in media containing predigested gluten or predigested gluten plus non-digested gluten. Passages were carried out every 24 h for 15 days in the same medium and community composition along time was studied via V3-V4 16S rDNA sequencing. Diverse microbial communities were successfully obtained. Moreover, communities were shown to be maintained in culture with stable composition for 14 days. Under non-digested gluten presence, communities were enriched in members of , such as , , , , or , and members of , such as and . Contrarily, communities exposed to digested gluten were enriched in . Hence, this study shows a method for culture and stable maintenance of gut communities derived from gluten metabolism. This method enables the analysis of microbial metabolism of gluten in the gut from a community perspective.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25074013