The role of 25-hydroxyvitamin-D3 and vitamin D receptor gene in human periodontal ligament fibroblasts as response to orthodontic compressive strain: an in vitro study

• Published in: BMC oral health 2021-08, Vol.21 (1), p.386-10, Article 386
• Main Authors: Küchler, Erika Calvano, Schröder, Agnes, Teodoro, Vinicius Broska, Nazet, Ute, Scariot, Rafaela, Spanier, Gerrit, Proff, Peter, Kirschneck, Christian
• Format: Article
• Language: English
• Subjects: 7-Dehydrocholesterol reductase; Alfacalcidol; Analysis; Binding sites; Calcifediol; Cell culture; Connective tissue; Cyclooxygenase-2; Fibroblasts; Gene; Gene expression; Genetic aspects; Humans; Hydroxylase; Interleukin 6; Ligaments; Observations; Orthodontics; Osteoprotegerin; Periodontal Ligament; Physiology; Polymorphism; Receptors, Calcitriol - genetics; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Software; Statistical analysis; Teeth; Tooth movement; TRANCE protein; Values; Vitamin D; Vitamin D receptors; Vitamin deficiency
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/s12903-021-01740-8

This study aimed to investigate, if different physiological concentrations of vitamin D (25(OH)D ) and single nucleotide polymorphisms in vitamin D receptor (VDR) gene have an impact on gene expression in human periodontal ligament (hPDL) fibroblasts induced by simulated orthodontic compressive strain. A pool of hPDL fibroblasts was treated in absence or presence of 25(OH)D in 3 different concentrations (10, 40 and 60 ng/ml). In order to evaluate the role of single nucleotide polymorphisms in the VDR gene, hPDL fibroblasts from 9 patients were used and treated in absence or presence of 40 ng/ml 25(OH)D . Each experiment was performed with and without simulated orthodontic compressive strain. Real-time PCR was used for gene expression and allelic discrimination analysis. Relative expression of dehydrocholesterol reductase (DHCR7), Sec23 homolog A, amidohydrolase domain containing 1 (AMDHD1), vitamin D 25-hydroxylase (CYP2R1), Hydroxyvitamin D-1-α hydroxylase, receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2) and interleukin-6 (IL6) was assessed. Three single nucleotide polymorphisms in VDR were genotyped. Parametric or non-parametric tests were used with an alpha of 5%. RANKL, RANKL:OPG ratio, COX-2, IL-6, DHCR7, CYP2R1 and AMDHD1 were differentially expressed during simulated orthodontic compressive strain (p