Acrosome reaction and chromatin integrity as additional parameters of semen analysis to predict fertilization and blastocyst rates

Traditional semen parameters have shown little to none predictive value for fertilization and blastocyst viability for a successful pregnancy. Therefore, the purpose of this study was to explore the usefulness of incorporating the acrosome reaction (AR) and chromatin integrity to conventional semen...

Full description

Saved in:
Bibliographic Details
Published in:Reproductive biology and endocrinology 2018-10, Vol.16 (1), p.102-102, Article 102
Main Authors: Tello-Mora, Pamela, Hernández-Cadena, Leticia, Pedraza, Jeimy, López-Bayghen, Esther, Quintanilla-Vega, Betzabet
Format: Article
Language:English
Subjects:
Acrosome reaction
Acrosome Reaction - physiology
Acrosomes
Adult
Andrology diagnosis
Blastocyst - cytology
Blastocyst - physiology
Chromatin
Chromatin - genetics
Chromatin - metabolism
Cross-Sectional Studies
DNA fragmentation
Female
Fertility - physiology
Fertilization - physiology
Fertilization in Vitro - methods
Genetic aspects
Humans
In vitro fertilization
Infertility, Male - genetics
Infertility, Male - physiopathology
Male
Middle Aged
Physiological aspects
Pregnancy
Semen
Semen Analysis - methods
Semen quality
Spermatozoa - metabolism
Spermatozoa - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Traditional semen parameters have shown little to none predictive value for fertilization and blastocyst viability for a successful pregnancy. Therefore, the purpose of this study was to explore the usefulness of incorporating the acrosome reaction (AR) and chromatin integrity to conventional semen analysis to individually predict the fertile potential of sperm samples. A cross-sectional study was conducted in 69 participants undergoing IVF using oocyte donation. Semen samples were collected and evaluated for: AR [spontaneous (sAR) and induced (iAR)] by flow cytometry using anti-CD46-FITC, Acrosome Response to an Ionophore Challenge (ARIC), chromatin integrity by Sperm Chromatin Structure Assay (DNA Fragmentation Index-%DFI and High DNA Stainability-%HDS), WHO semen analysis, fertilization and blastocyst rates. The participant age was 40.0 ± 6.1 years (66% were normozoospermic). Sperm morphology, sAR, iAR, and ARIC were associated with the fertilization (β = 3.56, R  = 0.054; β = - 5.92, R  = 0.276; β = 1.83, R  = 0.150; and β = 2.10, R  = 0.270, respectively, p 
ISSN:1477-7827
1477-7827
DOI:10.1186/s12958-018-0408-0